In this example Sample1.fcs is displayed on one plot and Sample2.fcs is displayed on another plot at the same time.

Those two data files belong to different iterations. If the user switches to the next iteration, the top plot would then display Sample2.fcs and the bottom plot would then display Sample 3.fcs.

If the user switches to the last iteration, the top plot would display Sample 7.fcs and the bottom plot would display Sample 8.fcs. Since there is no data file after Sample 8.fcs in the data list, it is not possible to have an 8th iteration. For this reason Iteration 8 is gray.

To resolve this issue:

• Set all of the plots to display the same data file. This can be done by:
  • dragging and dropping a data file from the data list onto the plot
  • or clicking on a file in the data list, and Change Data On All Plots.

When the same file is displayed on both plots, all 8 iterations are blue.

• Alternatively, deleting the plot displaying Sample 2 would resolve the issue.

In this example, multiple files are overlaid on a histogram or other plot.

In the example below, all 8 data files are overlaid on the histogram and are displayed simultaneously. Because there are no other data files in the data list to switch to during batch processing, Iterations 2-8 are gray.

In this case, if you do not want those overlays to change to different data files, simply set all of the overlays on the plot to not participate in batch processing.

In the Overlays category of the plot's formatting menu, highlight all overlays and uncheck Change data during Next/Prev/Batch.

In this example, statistics or keywords from multiple files are displayed simultaneously in an internal spreadsheet.

If the user drags and drops data files from the data list into an internal spreadsheet to report tokens, those tokens will participate in batch processing by default. For this reason, the number of possible iterations gets reduced.

If you do not want the reported tokens to participate in batch processing (changing from file to file):

• after dragging and dropping the data files from the data list, and selecting Statistic token or Keyword token,
• Click the Data Source option in the left pane
• Uncheck Allow the Data file of this token to change when running Next/Prev/Batch Processing

In this example, a data file that is not in the current iteration was dragged from the data list onto a Batch Action.

In this example, the current iteration is Iteration 1. Sample1.fcs is being displayed on the plot.

A data file in another iteration (Sample2.fcs) was dragged and dropped onto the Excel Batch Action.

This reduces the number of possible iterations, causing Iteration 8 to become gray.

To fix the issue:

• Delete that statistic token from the batch action.

To avoid this issue:

• only drag and drop a file in the current iteration onto a Batch Action.
• or drag and drop an object from the layout that is displaying a file in the current iteration.
  • (e.g. plot, a gate, a statistic, or a spreadsheet cell)