Why have percentages reported by quadrants changed after updating to FCS Express version 7.20.0020?
In FCS Express version 7.20.0020 and later, quadrants are converted to gates by default. The reasons for a possible change in reported percentages are:
- Quadrants no longer have a concept of out of range values.
- An event may be placed in a different quadrant than before.
1. Quadrants no longer have a concept of out of range values.
Previously, FCS Express had an option for indicating if out of range events were included in quadrant calculations. Events are "out of range" if they are outside the Resolution Minimum and Resolution Maximum. In version 7.20.0020 and later, the quadrant borders extend from the centerpoint to infinity and all events are included in a quadrant. If out of range events were excluded from quadrants in version, 7.18.0025 and earlier, and the same layout is opened in 7.20.0020 and later, you may notice a change in the reported percentage within quadrants. Consider the example below:
In FCS Express version 7.18.0025, in the Specific Options category of a plot's formatting menu, the option Include out of range values in quads was unchecked, and a Resolution Minimum and Resolution Maximum were specified. | The percentage of events in each quadrant is shown below. | When the layout is saved and opened in version 7.20.0020 or later, the quadrants automatically include out of range values. For this reason, the reported percentage of events in each quadrant is different than in Figure 1. Notice in Figure 2 below that the sum of the values is 100% while in Figure 1 the sum is 42.74. |
Figure 1 |
Figure 2 |
If out of range values were included in quadrants before updating to version 7.20.0020 and later, you may notice a slight difference in the reported quadrant statistics. The way those events have been assigned to a quadrant have been changed to be more accurate, as described below.
2. An event may be placed in a different quadrant than before.
This primarily affects events that are located along the border lines of the quadrants. Now that quadrants are gates in version 7.20.0020 and later, the events along the quadrant borders are assigned to individual quadrants in a different way. Any difference in the reported quadrant statistics due to the new assignments is expected to be small.
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Installation
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Licenses
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- Can I get more information regarding the Add-Ons that can be purchased with a license?
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- Does FCS Express have any features to help meet 21 CFR Part 11 compliance?
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- Can I convert my Cytek license from the countercode licensing option to another licensing option?
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Layouts & Loading Data
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Data Analysis
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- Caveats when using Biexponential Scaling with automatic Below Zero parameter detection in the presence of outliers.
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- When acquiring spectral data, should my single-stained controls be "as bright or brighter" than my fully-stained sample?
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- Can a set of quadrants be both percentile and floating?
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- Can I create an output file that contains the same plot from each data file on a single page?
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- How are statistics in FCS Express calculated compared to how they are calculated in BD FACSDiva?
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Image Cytometry
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- How do I adjust the axes to display small particle data from Amnis CellStream?
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FCS Express on Mac
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Upgrading FCS Express
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- Can different versions of FCS Express exist on the same computer?
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Clinical & Validation Ready
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- Can I get more information regarding the Add-Ons that can be purchased with a license?
- Can I lock my template based on an electronic signature?
- Does FCS Express have any features to help meet 21 CFR Part 11 compliance?
- What is the difference between the different types of Users that are available with a Security and Logging license?
- What is the difference between the Logging option and System Level Audit Trails?