What is UMAP?

Uniform Manifold Approximation and Projection, or UMAP, is a dimensionality reduction technique that allows users to create new UMAP X and UMAP Y parameters from a high-dimensional dataset.

UMAP is now available in FCS Express through its new Pipelines feature.

Advancements in cytometry have allowed for researchers to capture information for many different parameters on a single cell.  Analyzing the complex high-dimensional single-cell data can present many challenges.  Many algorithms have been created to assist in understanding and visualizing these data sets.

In FCS Express, we offer UMAP (Uniform Manifold Approximation and Projection) algorithm as a Pipeline step.  UMAP is a dimensionality reduction technique that constructs a high dimensional graph representation of the data then creates a low-dimensional graph to be as structurally similar as possible. This results in the creation of two new parameters UMAP 1 and UMAP 2.  UMAP captures local relationships within a cluster as well as global relationships between distinct clusters.

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