FCS Express Knowledge Base Filter by categories Clear Results Data Analysis Exporting High Dimensional Layouts Loading Data Plots Statistics Transformations FCS Express on Mac Mac Installation Working in the Mac Version Image Cytometry Amnis IDEAS Data Other Image Cytometry Installation Configuration Installing the Application Network License System Specifications Licenses Add-ons Claim Codes Editions (RUO & IVD) License Types Previous Versions (Flow, Image & Plus) and Versions Consolidation Subscription Upgrading FCS Express Changes in the new version Transitioning from previous releases Installation Installing the Application Do you offer an IQ/OQ program to be used as part of our validation? How can I do a silent installation? How do I install the FCS Express demo? How do I reinstall FCS Express on another computer? What do I choose for the .FEY Extension Registration Question? Network License How do I run 32-bit and 64-bit versions of the software for the same network license? What are the details for the network architecture of FCS Express? Why do I get an error about copying FCSExpressApp.exe? Configuration How do I manually change my configuration file? What is a configuration file? What is a Countercode? Where is the configuration file located on my computer? System Specifications How much memory (RAM) can FCS Express use? What are the system requirements for FCS Express? Licenses Add-ons Can I get more information regarding the Add-Ons that can be purchased with a license? Can I lock my template based on an electronic signature? Does FCS Express have any features to help meet 21 CFR Part 11 compliance? Does FCS Express have Quality Control features? Does FCS Express offer Single Sign On capability? How do I configure SQL Server to host a database for FCS Express? What database options are available when I purchase the Security option? What is the difference between the different types of Users that are available with a Security and Logging license? What is the difference between the Logging option and System Level Audit Trails? What SQL Server permissions are needed? License Types Internet Dongle & USB Dongle Can I share my USB dongle or countercode license with another user? Can I track usage of the internet dongle? Can I try out the Internet Dongle before I make a purchase? Can the administrator log users out? Do you have to be connected to the internet at all times with the Internet dongle? How can users be added to an internet dongle license? How do I activate my dongle? How do I change my internet dongle/site license password? How many people can be logged in at the same time? How many user accounts can I create? If a user left the computer running can the user log themselves out from another computer? What are the differences between the internet dongle and network licensing options? What happens if I lose my internet connection? What happens if the user leaves the computer without logging out? What happens to the users login in case of an unexpected interruption? For instance, a software crash, power failure, etc. Why am I receiving a message that FCS Express cannot connect to De Novo Software servers? Network & Countercode How do I update my network license after renewal? Site Licenses How are site licenses billed? How do you calculate the number of site license users? How many people can be logged into the site license at the same time? How many user accounts can I create on the site license? Demo How can I extend my FCS Express Demo? How do I convert my demo to a purchased license? How do I switch from the FCS Express Reader to the demo? What is the difference between the demonstration and purchased versions? Why am I unable to save my new configuration file when extending my demo? Claim Codes Can I convert my Cytek license from the countercode licensing option to another licensing option? How can I claim my license purchased through BD Accuri Cytometers? How can I claim my license purchased through BD Biosciences? How can I claim my license purchased through Nexcelom Biosciences? How can I claim my license purchased through Sysmex-Partec GmbH? How can I claim the FCS Express license that came with my Cytek instrument purchase? Editions (RUO & IVD) Do you offer a clinical Image Cytometry Version? What features are found in FCS Express Clinical Edition? What is the difference in features between the Flow, Image and Plus Versions? Subscription Can I renew my subscription online? How can I get an estimate? What is your refund policy? Why is FCS Express 7 only offered via a yearly or monthly subscription rather than “perpetual”? Previous Versions (Flow, Image & Plus) and Versions Consolidation FCS Express 7 Versions Consolidation How do I know which version of FCS Express I have? Data Analysis High Dimensional How can I create a merged data with equally-sized downsampled samples? How can I do pre-processing for high-dimensional data analysis? How can I explore tSNE/UMAP plots? How do I use SPADE? What is FlowSOM? What is T-SNE? What is UMAP? Transformations Can FCS Express integrate Python scripts? Can I use the FlowAI script in FCS Express? Can I use the FlowClean R Script with FCS Express? How can I recreate ratiometric data acquired in FACSDiva? How do I use R Integration with FCS Express? How does FCS Express implement software compensation? If my data does not have a Time parameter, can I create one? What is compensation? What is the compensation workflow in FCS Express? Plots Can I customize the display of my data from different instruments? Can I disable the live updating feature? How can I display all of my detectors for my Cytek data? How can I set FCS Express so my FCS 3.0 biexponential data looks the same as it did in the BD FACSDiva software? How do I display Summit data in FCS Express as it appears in the Summit Software? How do I fix the biexponential axes on a plot? How do I rescale CytoFLEX data so it displays as it did at acquisition? How do I update my density and contour plots created in Version 4 to use the newest color palette? What are resolution options? What is Biexponential and Hyperlog Scaling? What is the best way to set FCS Express to display FCS 3.0 data from FACSDiva on a 4 decade log scale? Where can I get more information regarding DNA analysis using the Multicycle AV? Why can’t I change my plot axis labels from the Name keyword to the Stain keyword? Why do my dot plots appear sparse and blocky? Why is the text on the right most label cut off my plot? Exporting Can I create an output file that contains the same plot from each data file on a single page? How do the batch processing run modes differ, and why would I use them? Why do I get an “Old format or invalid type library” error when using Microsoft excel during batch analysis? Layouts Can I customize my Quick Access Toolbar? For an existing layout, can I change the way FCS Express remembers parameters? How do I change my layout from portrait to landscape? How do I change the resolution of my exported images? How do I re-establish data files that were linked to a layout? Statistics How are statistics in FCS Express calculated compared to how they are calculated in BD FACSDiva? How can I display my statistical data in Scientific Notation? How do I calculate EC/IC Anything? What is “Stain Index” and how do I calculate it with FCS Express? What is MFI (Mean or Median Fluorescence Intensity) and how do I calculate it in FCS Express? Why is the Geometric Mean being reported as NaN or ##ERROR##? Loading Data Are Beckman Coulter LMD files unique? Can I find a support resource page for the analysis of Cytek data in FCS Express? How can I easily create the "filename" column in the "ExtraKeywordsTable.csv" file? How can I load data from the BD Accuri C6 Flow Cytometer? How do I change the display in my plots from one data file to another data file? How do I export .ICE files from Thermo Cellomics HCS Studio? How do I tell FCS Express what plate size to use if that information is not included in the data file? How do I upload files to the De Novo Software FTP site? How do I use BD Accuri CFlow files with Multicycle DNA analysis in FCS Express? How do I work with images from the Thermo Scientific Attune CytPix? What is the Elapsed Time setting in the Gallios software and how do I convert it to real time? Why are there sometimes access violations when I save and load files? Why do I get the message that a data file exported from a FACSDiva™ Experiment is invalid? Image Cytometry Amnis IDEAS Data How do I adjust the axes to display small particle data from Amnis CellStream? How do I choose which images and parameters to view in a Data Grid? How do I export/save data from IDEAS software and load it in FCS Express? How do I make my images in the data grid larger? How do I pseudo-color images in a data grid? How do I work with Amnis derived image cytometry data in FCS Express? Other Image Cytometry Can I display heat maps with my Image Cytometry data? Can I work with data from PerkinElmer Instruments? Do you offer 21 CFR Part 11 compliance options for the Image Cytometry Version? Do you offer image segmentation or image analysis? How do I use CellProfiler Data with FCS Express? How do I use ImageJ with FCS Express? What file formats are compatible with FCS Express Image Cytometry? Where can I find Nexcelom Resources and Applications? FCS Express on Mac Mac Installation Can FCS Express run natively on Mac? How do I install FCS Express 6 on a Mac? I want to run FCS Express 6 or 7 on a Mac and a PC. Will I need to purchase two separate licenses? Where is my configuration file saved on my Mac? Which macOS are supported by FCS Express 6 and 7? Working in the Mac Version Can FCS Express integrate R scripts on a Mac? Can FCS Express layouts made on a Mac be opened on PC (and vice-versa)? The Data List and other dockable windows are missing on my Mac. How can I fix this? Why does FCS Express appear frozen when I create a gate? Upgrading FCS Express Changes in the new version Where did Edit Preferences go? Transitioning from previous releases Can different versions of FCS Express exist on the same computer? How can I view and convert my V3 layouts to FCS Express 7? How do I import my version 3 security databases into newer versions of FCS Express? How do I update Density Plots created in Version 4? Is there an upgrade discount from earlier versions of FCS Express? Why are my density plots from V3 not displayed correctly in later versions? Why are there fewer outlier dots on my FCS Express 5 and later density plots than in V4?