• Open the plot's format menu
• Navigate to the Axes category of the Format menu
• Select the X or Y axis button
• Uncheck Automatic Minimum and/or Automatic Maximum, and specify a custom Minimum or Maximum for the axis.
• Pick a value such that all events in the population of interest appear within the plot area.
• Do the same for the other axis, if desired.

• Once all events are visible on the plot, resize the gate to include the entire population of interest.

• Optional: On this plot, display other data files in the dataset to ensure the gate is in the desired position for all affected data files.
• Adjust the Axis minimum and maximum back to how they were earlier, or how they are preferred for presentation.

• Right-click on the plot and select Duplicate

• Open the format menu for the new plot. Navigate to the Axes category of the format menu
• Click the X or Y axis button
• Under the Min/Max Resolution section set a custom Minimum and/or Maximum value for the axis. Pick a value such that all events in the population of interest appear within the plot area.

• Do the same for the other axis, if desired.
• Resize the gate to include all events in the population of interest.

• Optional: On this plot, display other data files in the dataset to ensure the gate is in the desired position for all affected data files.

• Once the gate position has been finalized, delete the plot copy- it is no longer needed.

The borders of quadrants extend to infinity. If you wish to create a gate with borders that extend to infinity, you may add a set of quadrants to the plot and create a linked gate. This creates a gate which shares the same borders as a single quadrant.

• Click Gating tab > Quadrants
• Click on the plot of interest to add a set of Quadrants
  • Click OK to use the default naming scheme, or enter custom names.
• Click the centerpoint of the quadrants, click the Format tab > Selected Gate or Quads
• Under the Angles section of the format menu, Checkmark the checkbox called Floating if you want the borders of the gate to be angled.

• Create a linked gate for one of the quadrants by clicking Create under the section called Linked Quad Gates
  • Enter a name for the new gate
  • Select which of the 4 quadrants to link to the gate
  • Select a Parent gate for the new gate
  • Click OK

• The dimensions of the new gate are controlled by the Quadrant position. It is recommended to keep a copy of this plot (showing the quadrants) in the gray space of the layout so you can make adjustments as needed.
• You may hide the quadrants from a plot by clicking on the centerpoint, pressing the Delete button on your keyboard, and clicking Hide
• To show the gate on a plot:
  • Right-click on the plot, choose Gates > Show gates
  • Checkmark the gate that is linked to the quadrant, and click OK