ASCLS P.A.C.E.® CE available

Spectral Flow Cytometry in the Clinical Laboratory Setting

November 5, 2024 2:00pm EST/1:00pm CST

Presented by:

Paul E. Mead, Ph.D., SCYM(ASCP)

Principal Scientist - Immunopathology, Department of Pathology

St. Jude Children's Research Hospital

Space is limited - Register here today!

Spectral flow cytometry is ideally suited for high-parameter flow cytometry applications in the clinical setting.  The ability to include many markers in the same tube provides several advantages including simplifying data analysis and gating strategies while using significantly less sample volume when compared to multi-tube conventional flow panels.  This is particularly important in the minimal residual disease (MRD) setting, where cell numbers can be low following intensive chemotherapy and where the cells available may need to be split between various testing modalities.  Here, we will discuss the development of a high-parameter single-tube panel for immunophenotyping pediatric leukemia.  This 38-parameter tube has recently replaced a 13-tube, 8-colour conventional immunophenotyping panel in our flow cytometry work up of new, or relapsed, pediatric leukemias.  We will discuss the development of the panel and the validation process in the clinical laboratory, with particular attention to steps in the validation process that were unique to spectral applications.

Level of Instruction: Advanced

Objectives:

• Review the fundamental differences between spectral and conventional flow cytometry.
• Discuss the strategies involved in high-parameter panel design.
• Outline the steps involved in validating a spectral panel for clinical implementation.

Contact Hours: 1 contact hour

Program Number: 231-001-24

Space is limited - Register here today!

De Novo Software is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program