How do I rescale CytoFLEX and DxFLEX data so it displays as it did at acquisition?

• By default, when a plot is created and a data file is loaded on to the plot, the axis minimum and maximum are set according to the Instrument Specific Settings.
• These are found in File tab > Options > Data Loading > FCS File Options > Instrument Specific Settings
• FCS Express reads the $CYT keyword and applies the associated ISS automatically when data files are loaded into a layout.

• To review or edit the ISS for CytoFLEX data, select CytoFLEX from the list of instruments. Or if your data are from the DxFLEX, choose DxFLEX from bottom of the list.
• Axis Minimum, Maximum and scale can be set independently for Scatter parameters (e.g. FSC and SSC) and Fluorescence parameters (e.g. APC, FITC)
• You can see in the screenshot below that for CytoFLEX data, the default axis Minimum and Maximum are Based on Data for scatter parameters.
  • This means the axis minimum and maximum will be set such that all data points are visible on the plot (even events that are outliers or noise). i.e. The axis minimum and maximum are equal to the lowest and highest data point values respectively.

• Within that ISS menu, if the Manual option is selected, the user can specify a minimum value and a maximum value to be used by default on all plot axes displaying Scatter parameters (see the screenshot below for an example).

• When a plot is selected and Automatic Minimum or Automatic Maximum is checkmarked, the value is sourced from the Instrument Specific Settings (described above).
• If you would like to set a custom Minimum or Maximum for just the selected plot(s), uncheck Automatic Minimum or uncheck Automatic Maximum and enter the desired custom value.

FCS Express also allows you to easily zoom to the portion of the plot that contains your relevant data so you can get results quickly. Simply right click on any plot and choose Magnify (Resolution). Draw a region around the portion of the plot to zoom to.