About De Novo Software

De Novo Software has been developing flow cytometry data analysis solutions since 1998. Over the last 20+ years FCS Express has become a world-renowned as a robust and easy to use data analysis application. Our consulting division has vast experience in implementing data analysis solutions, both in flow cytometry and image cytometry related fields. 

De Novo Software has a strong cast of talented and creative developers who work hand in hand with our applications, sales, support, and operational teams. The close collaboration of our flow and image cytometry experts with programmers helps make FCS Express an exceptional tool for research and clinical professionals.

De Novo Software works closely with many industry leading companies and partners to provide unparalleled software support and integrations for flow and image cytometry data analysis and compliance. Additionally, an international network of distributors helps make purchasing FCS Express surprisingly local.

Contact our team with any questions you may have.

De Novo Software is a rapidly growing company specializing in data analysis solutions for scientific experiments based in Los Angeles California. Our flagship product, FCS Express, is used in thousands of research and clinical laboratories world-wide in applications ranging from basic science to disease diagnosis.                                                                                                                                                                                             

De Novo Software is an active member of the flow and image cytometry community and thousands of researchers around the world publish with FCS Express. Please see our short list of Flow Cytometry Publications and Image Cytometry Publications for examples of how research and clinical researchers use FCS Express to get results and publish. 

De Novo Software maintains close relationships with various e-learning courses, authors, and companies that provide educational resources related to FCS Express for the cytometry community.


"FCS Express has revolutionized the way we think about data analysis. Not only are ALL the analysis tools available at your fingertips, the ability to work in a “Microsoft-like” environment makes the whole process intuitive and a great time savings. Our users have shared the same level of enthusiasm about our decision to switch to FCS Express for all our data analysis needs.”

Joanne Lannigan Ph.D.
Director, Flow Cytometry Core
University of Virginia 
School of Medicine

"FCS Express is the most intuitive, flexible, and comprehensive flow cytometry software we have ever used. The company is extremely responsive and supportive with helping us to accomplish very complex analyses and make sure the software is meeting our clinical laboratory needs. We have gone through several instrument and LIS transitions over the past 10 years and have been able to rely on the software to help alleviate the pain of those changes. We would highly recommend this for any flow lab!"

Carrie Gifford
Business Manager, Cancer & Blood Diseases Institute
Clinical Laboratories

"We have been offering FCS Express to our shared resource lab users since 2017. Thanks to the excellent support provided by De Novo Software, we have had a steady increase in users adopting this platform. De Novo Software is ready with classes, phone and online support and fits in well with the research services environment. Our users particularly like the user friendly interface!"

Jonni S. Moore Ph.D.
Professor and Faculty Director
Abramson Cancer Center Flow Cytometry and Cell Sorting Shared Resource
Perelman School of Medicine at University of Pennsylvania


“FCS Express from De Novo Software is an excellent software suite to facilitate researchers in their varying experimental complexities for flow and image cytometry.  The functionality and stability of the software combined with excellent and timely support has made it an important tool for research cytometry at TSRI"

Alan Saluk
Sr Scientific Director, Flow Cytometry
The Scripps Research Institute


"The customer service, and the functionality of the software is by far the best I’ve seen. Unless forced to, I will never touch FlowJo again"


"You guys have the best customer service of ANY product I own."

David Grier

Wake Forest School of Medicine

"My users love the interface of FCS Express and find it quick and easy to learn. We love the speed and flexibility with data analysis. As a core manager, I appreciate the support the FCS Express team provides; they are always available to help my users." 

Vanessa Scarfone
Flow Cytometry Core Manager
Stem Cell Research Center
University of California Irvine

"Our group uses a wide variety of software for data analysis and processing. We are committed to using FCS express for our flow cytometry data needs as it is the most intuitive and flexible software available. The ease in interfacing with other Windows-based software and a convenient multiuser network installations were major determinants in our decision to purchase FCS Express. My staff enjoys being able to log in and process data from any desktop computing system in our facility. Application support and accessibility to a network of FCS Express users has also been very useful in developing batch processing and production protocols. Overall, I have not experienced the ease of use and broad applicability with other flow software product."

David DiGiusto, PhD

"I have found the online support material has been really helpful to my students to extend their knowledge independently."

Terry Pinfold

Cytometry and Histology Lab Manager, University of Tasmania

"I can't say enough good things about the program and the people who work on it."

Lisa Kohli
Laboratory Technician

"My function here at Integrated Oncology is to set up a work flow process for over 350 cases a day companywide so without a doubt I am choosing FCS Express to accomplish this. In my opinion there just isn't another software option for us. I need a user friendly package that will accommodate our current complex multicolor analysis as well as be able to expand for future growth, provide high quality reports for our clients, offer a virtual component for those customers that want to offer tech only flow or more closely interact with their patient results and the big challenge is that this has to happen in an extremely high volume reference laboratory. De Novo Software has been able to provide us with the features that we need and their technical support is extraordinary. I can't imagine a successful clinical lab without FCS Express; it is the "go-to" Clinical package."

Horacio Vall CLS, SCYM(ASCP)
Vice-President Science & Technology
Doctor of Biochemistry
Flow Cytometry Discipline Director Laboratory Corporation of America Holdings

"A major decision in our choice of software packages was dictated by how well we thought it would integrate with our report writing software (MS Word.) It is my impression that FCS Express does a better job with interfacing with other widely used software programs than the two other programs that we evaluated. It is hard to put into words, but FCS Express also seemed to have the look and feel of what I thought a "clinical" software program should be. Currently we spend excessive time in clerical tasks of transferring numerical values into our reports. In comparison with the other programs we reviewed, FCS Express seems to better suited to handle these repetitious tasks that are a big part of our clinical work and help separate this application from the needs of a "research" user. In short, the "automation" features of FCS Express seem stronger which are important to our clinical laboratory. Currently there is a labor tight market for qualified personnel in our area and we find it hard to attract technicians. Moreover it often takes years to fully train a technician. By using FCS Express anticipate shortening the training cycle by allowing our technicians more time to focus on the more demanding tasks in flow cytometry and less on clerical busy work. We are currently using DiVa, which is a great data acquisition program, but it does have serious limitations when used for routine data analysis and report generation thus leading to our decision to purchase FCS Express."

Nick Gonchoroff
Retired Associate Professor
Suny-Upstate Medical University

"The Hematopathology laboratory of the Shands Hospital at the University of Florida analyzes numerous bone marrow, blood and lymphoid tissue samples for the diagnosis of leukemia, lymphoma and related neoplastic conditions every day. We have been using BD's software for our data acquisition and analysis for many years. While adequate for acquisition, this software has several limitations regarding data analysis that recently prompted us to look for alternative programs for our clinical analytical work.   De Novo Software has provided an analysis tool that is completely optimized for clinical laboratories like ours. Within a short period of time, we created a drastically improved analysis protocol in FCS Express and it seems that every day we discover new features, such as tokens, alerts, panels, etc. that easily allow us to improve the quality of the analysis. Above all, I am extremely impressed with the excellence of the support staff that is always available to answer questions and courteously and positively responds to suggestions. In my opinion, FCS Express is one of the best programs available today for clinical flow cytometry laboratories."

Raul Braylan
Former Medical Director, Flow Cytometry Laboratory of the Shands Teaching Hospital at the University of Florida 

"I used to be in flowjo camp but I'm now totally converted to FCS express camp! Loving your software"


"I am reaching out to let you know that V7 has been a great experience. My analysis time has been chopped by more than 50%. All the new features, etc, are great!"

Alberto Robert