The De Novo Software Team

David Novo PhD, Founder
David received his PhD in Biophysics from UCLA. His research concentrated on developing novel optical and mathematical techniques for measurements and analysis of localized fluorescent Calcium transients from a microdomain within a muscle cell. David also built several confocal microscopes, as well as writing the data acquisition and data analysis systems. Prior to commencing his PhD, David worked with Dr. Howard Shapiro, one of the leaders in the field of flow cytometry, performing research on membrane potential determination in bacteria using flow cytometry. During the course of this research they built several flow cytometers custom made for bacterial measurements. Two papers were published on the subject. David still maintains close ties to the flow cytometry community through his appointment to the Data Standards Committee of the International Society of Analytical Cytometry. This committee maintains the flow cytometry data file standard and deals with issues standardizing all sorts of flow cytometry related data storage and analysis issues. David frequently consults on data analysis issues related to flow cytometry and collaborates on many projects involving novel data analysis requirements.

Tanya Tolmachoff MLS(ASCP)SI Director of Clinical Sales and Marketing
Tanya brings many years of cytometry based experience with her to De Novo Software. She learned flow cytometry at Cedars Sinai Medical Center in the Division of Immunology & Cytometry working as a licensed Medical Technologist.  While at CSMC, Tanya earned her ASCP certification as a Specialist in Immunology, performed didactic lectures for the School of Med. Technology, functioned as the Immunology Division's Pathology Resident Teaching Facilitator, and was the primary instructor for a CAP approved Practicum covering Image Analysis & Flow Cytometry DNA analysis techniques.  Focusing on image analysis, she co-authored several scientific papers as well as a chapter in Image Analysis: A Primer for Pathologists.   Tanya joined Beckman Coulter Inc. as the Southern California Cytometry Accounts Manager and later became the Cytometry Systems Specialist for the South Western Region covering CA, AZ, NM, TX, and HI.     Tanya graduated Magna Cum Laude with a BS in Microbiology from California State University, Fresno.

Daniel ZimmermanChief Technical Officer
Since getting his start in the computer science program at McGill University, and in the IBM development lab in Toronto, Daniel has been with De Novo Software, writing code, guiding program design, and overseeing the extensive testing infrastructure and quality systems.

Sean Burke, President
Sean has worked with the team at De Novo Software since 2010 starting his career with FCS Express as a technical application specialist, moving on to product management and transitioning into director of commercial operations prior to his recent roll. He received his M.S. degree in Biology from New York University where he utilized flow cytometry to study interactions between multiple HIV-1 viruses within single cells and acted as operator of the College of Dentistry Flow Cytometry Core facility. Prior to NYU, he worked at Memorial Sloan-Kettering Cancer center developing methods for automated microscopic analysis of tissue sections at the single-cell level. Over the years he has acquired wide range of experience with flow cytometry, image analysis, business development and enjoys working closely with FCS Express customers, partners, and our team of talented developers, scientist and staff.

Juan Vazquez, Manager - Customer Support
After receiving his ASCP certification, Juan worked at Brigham and Women's Hospital in Boston. He then joined BD Biosciences as a Technical Applications Specialist, helping clinical and research customers with their flow cytometry-related needs. His experience and enthusiasm are tremendously popular with FCS Express customers.

Hemant Agrawal PhDTechnical Applications Specialist
After obtaining his Ph.D. in Immunology from the University Hospital Essen, Germany he later joined OMRF, Oklahoma City as an Associate Research Scientist in the Immunology and Arthritis Program. Thereafter, he was recruited as a Research Associate Scientist at the Rheumatology Department at Northwestern Memorial Hospital, Chicago. He then joined TreeStar, Inc as an application support scientist for the Indian subcontinent and Middle, helping customers with data analysis. Dr. Agrawal is also the director of Flowcytometry Solutions Pvt Ltd, an organization, which imparts training and consultancy in the field of flow cytometry. Over the years, he has gained experience in flow cytometry data acquisition and analysis and will be providing technical support and training to customers in the India region.

Andrea Valle PhDProduct Manager
Andrea is based in Italy and received his Ph.D. in Cellular and Molecular Biology from San Raffaele Hospital in Milan Italy in 2014 which was followed by an M.S. degree in Statistics for Genomics and Medicine. Andrea has worked closely with flow cytometry data analysis and experiments in a variety of settings since 2008 while working on immune tolerance in Type 1 Diabetes. Over the years he has gained additional skills in data analysis and R programming through his post-graduate studies and laboratory work.

Robin KamuchekaTechnical Applications Specialist
Robin graduated summa cum laude from the Honors College with a BS in Biology from the University of Texas-San Antonio. She brings many years of flow and image cytometry experience to De Novo Software. She has designed multiple experimental protocols for blood samples generated from human and animal research. Prior to cytometry work, she studied Chromosome 18 abnormalities and genetic expression in humans and Arabidopsis plants. Now at De Novo Software, Robin uses her unique background to partner with FCS Express customers to meet their data analysis objectives.

Holly Bonar MS, SCYM(ASCP)cmTechnical Applications Specialist
Holly graduated from Thomas More College with a BA in Biology in 2000, followed by an MS in Microbiology and Immunology from the University of Cincinnati College of Medicine in 2003.  Her master's degree thesis involved using flow cytometry to study the immune response to Histoplasma capsulatum infections in mice.  In 2007, she transitioned to the clinical flow cytometry laboratories of Cincinnati Children's Hospital Medical Center, where she spent 14 years performing and developing clinical flow cytometry testing to support the clinical services of the Cancer and Blood Diseases Institute.  Her flow cytometry clinical expertise includes a wide variety of adult and pediatric testing including leukemia/lymphoma immunophenotyping, minimal residual disease testing, and high resolution PNH screening, in addition to platelet function testing and hemoglobin F quantification for sickle cell anemia treatment.  She received her ASCP certification as a specialist in Cytometry in 2014 and has also served as a College of American Pathologists (CAP) laboratory inspector since 2015.

Greg Veltri PhD, Technical Application Scientist
Greg has 30 years’ experience in Flow Cytometry, Spectral, and Imaging. He received a BS and PhD in Biochemistry with concentrations in Cell Molecular Biology and Immunology. Through out the years Greg’s research has concentrated on AIDS, Cancer, Stem Cell, and Immunological diseases. Greg has had posts at UCSF, University of Minnesota, CRUK /University of Cambridge, and National Jewish Health. In addition, he has also worked in the private sector at BD Biosciences and Sony Biotechnology. Greg has work worked with multiple series of instrumentation, and software throughout the years , which he uses to provide technical support and training for De Novo Software.

Katherine Rittenbach MS SCYM(ASCP)cm, Technical Applications Specialist
Katherine studied Biomedical Engineering at Stevens Institute of Technology and Johns Hopkins University. She began her career at Vasculogic, Abbott and Becton Dickinson where she gained industry experience with In Vitro Diagnostics. Before joining the De Novo Software team, Katherine was a Research Specialist in the Princeton University Flow Cytometry Resource Facility. In addition to operating BD and Bio-Rad instruments, she provided Flow Cytometry training to Princeton researchers and used FCS Express to analyze diverse data sets. Katherine holds the ASCP(SCYM) certification and currently assists both new and experienced FCS Express users with training and troubleshooting.

Jamie McCarthy, Technical Applications Specialist
Jamie is based in England and he joined the De Novo Software team after previous experience as a Research Scientist for the Translational Sciences team at Quell Therapeutics, a Cell Therapy company in London. In this role he was responsible for the design, validation and execution of Flow Cytometry panels in a pre-clinical and clinical environment across a range of instruments. He had previously worked at the University of Leicester, in the Respiratory Sciences department, where his research focused on characterizing rare immune subsets. His academic background is in Immunology, having received both his Undergraduate degree and Master’s degree in the subject from University College London (UCL) and King’s College London, respectively. .


...and a strong cast of talented and creative developers based in our Pasadena, CA headquarters.

If you are interested in joining our team, please check for available positions on our careers page.