Can I customize my Quick Access Toolbar?
The FCS Express interface can be customized to allow quick access to the most common tools that are used for your analysis.
This can be done in two ways:
Please note that for macOS users, the Quick Access Toolbar is not available. Users will only be able to customize the Ribbon. For directions, click here.
The customization tool can be accessed by clicking on the downward triangle in the upper left of an FCS Express layout.
Customize the Quick Access Toolbar
When the Custom Quick Access Toolbar option is selected, the following window appears:
This window allows the user to choose commands that can be added directly to the Quick Access Toolbar. The user can find current commands that are located within the Ribbon, or, commands that are not located in the Ribbon, such as many of the commands that are found from the File tab. The user can add as many commands in the Quick Access Toolbar that can they can fit.
To add a command to the Quick Access Toolbar, the user simply needs to:
- Find the command, either from All Commands, a specific tab on the Ribbon, or a command that is currently not in the Ribbon.
- Click on the right-pointing triangle to add the command to the Quick Access Toolbar.
If a user wants to remove a command from the Quick Access Toolbar, they can simply select the command from the list of commands that have been added to the Quick Access Toolbar and then click on the left-pointing triangle. The user can also right click on the command and choose Remove.
Also, the user can choose to view the Quick Access Toolbar below the Ribbon by selecting the action from the Quick Access Toolbar menu.
Any customizations that are done to the Quick Access Toolbar can be reset by clicking on the Reset option in the lower left corner of the Quick Access Toolbar Customization window.
Customize the Ribbon
The user can also customize the Ribbon by:
- putting new commands onto existing tabs, or,
- creating new Tabs or Groups and adding commands to those tabs or groups.
From the Customize Quick Access Toolbar menu, select Customize the Ribbon. In this window, you can add commands to existing tabs or groups. You can also create your own tab or group.
A Group is a section on a tab. For example, the current Home tab is broken down into the groups Clipboard, Pages, Font, and Editing.
When the Ribbon Customization window opens, the concept is similar to how the Quick Access Toolbar is formatted.
The user will find the command that they would like to add, either from an existing tab on the Ribbon, or, a command that is not currently within the Ribbon.
If the user would like to add a command to an existing tab or group, they can:
- Select the command on the left.
- Select the tab or group to which they would like to add the command.
- Click on the right-pointing triangle to add the command.
To create a New Tab or Group, click on the Add button, then on New Tab or New Group.
A New Tab and Group can be renamed and moved to a different spot in the Ribbon.
Also, you can right click on the New Tab and Group to rename, remove or move.
Finally, once everything has been customized within the Ribbon Customization window, click OK to enable the customization.
The user can reset any customization with the ribbon by selecting the Reset option that is seen in the lower left corner of the Ribbon Customization window.
In macOS, there is no Quick Access Toolbar to customize. However, you can customize the Ribbon by creating new tabs and groups similar to as seen above.
As seen in Windows, you can customize the Ribbon from the menu at the top of the FCS Express layout.
When the Ribbon Customization window opens, the user can:
- Select the command to be added to an existing or new tab or group.
- Add a New Tab or Group.
- Select the Tab and Group to which the command will be added.
- Click on the right-pointing triangle to add the command.
- Use the up or down arrows to change the location of the tab and group.
- Click OK to enable the customization.
The user can reset any customization with the ribbon by selecting the Reset option that is seen in the lower left corner of the Ribbon Customization window.
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Installation
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Licenses
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- Can I get more information regarding the Add-Ons that can be purchased with a license?
- Can I lock my template based on an electronic signature?
- Does FCS Express have any features to help meet 21 CFR Part 11 compliance?
- Does FCS Express have Quality Control features?
- Does FCS Express offer Single Sign On capability?
- How do I configure SQL Server to host a database for FCS Express?
- What database options are available when I purchase the Security option?
- What is the difference between the different types of Users that are available with a Security and Logging license?
- What is the difference between the Logging option and System Level Audit Trails?
- What SQL Server permissions are needed?
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- Can I share my USB dongle or countercode license with another user?
- Can I track usage of the internet dongle?
- Can I try out the Internet Dongle before I make a purchase?
- Can the administrator log users out?
- Do you have to be connected to the internet at all times with the Internet dongle?
- How can users be added to an internet dongle license?
- How do I activate my dongle?
- How do I change my internet dongle/site license password?
- How many people can be logged in at the same time?
- How many user accounts can I create?
- If a user left the computer running can the user log themselves out from another computer?
- What are the differences between the internet dongle and network licensing options?
- What happens if I lose my internet connection?
- What happens if the user leaves the computer without logging out?
- What happens to the users login in case of an unexpected interruption? For instance, a software crash, power failure, etc.
- Why am I receiving a message that FCS Express cannot connect to De Novo Software servers?
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- Can I convert my Cytek license from the countercode licensing option to another licensing option?
- How can I claim my license purchased through BD Accuri Cytometers?
- How can I claim my license purchased through BD Biosciences?
- How can I claim my license purchased through Nexcelom Biosciences?
- How can I claim my license purchased through Sysmex-Partec GmbH?
- How can I claim the FCS Express license that came with my Cytek instrument purchase?
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Layouts & Loading Data
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- Are Beckman Coulter LMD files unique?
- Can I find a support resource page for the analysis of Cytek data in FCS Express?
- How can I easily create the "filename" column in the "ExtraKeywordsTable.csv" file?
- How can I load a Sony MA900 Index Sort file into FCS Express?
- How can I load data from the BD Accuri C6 Flow Cytometer?
- How can I load MACSQuantify files that were exported from MACSQuantify software version 3.0.1?
- How do I change the display in my plots from one data file to another data file?
- How do I export .ICE files from Thermo Cellomics HCS Studio?
- How do I tell FCS Express what plate size to use if that information is not included in the data file?
- How do I upload files to the De Novo Software FTP site?
- How do I use BD Accuri CFlow files with Multicycle DNA analysis in FCS Express?
- How do I work with images from the Thermo Scientific Attune CytPix?
- What is the Elapsed Time setting in the Gallios software and how do I convert it to real time?
- Why are iterations in my Data List gray?
- Why are there sometimes access violations when I save and load files?
- Why do I get the message that a data file exported from a FACSDiva™ Experiment is invalid?
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- How are existing quadrants handled when an old layout is opened in version 7.20 and later?
- How can I set quadrants to behave like conventional gates?
- How can I set quadrants to behave like in earlier versions?
- Quadrants in FCS Express versions 7.20 / 7.24 and later
- Why does the Quadrants Options window appear when I open an older layout in version 7.24?
- Why have percentages reported by quadrants changed after updating to FCS Express version 7.20.20?
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Data Analysis
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- Caveats when using Biexponential Scaling with automatic Below Zero parameter detection in the presence of outliers.
- How can I create a merged data with equally-sized downsampled samples?
- How can I do pre-processing for high-dimensional data analysis?
- How can I explore tSNE/UMAP plots?
- How do I use SPADE?
- What is FlowSOM?
- What is T-SNE?
- What is UMAP?
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- Can FCS Express integrate Python scripts?
- Can I use the FlowAI script in FCS Express?
- Can I use the FlowClean R Script with FCS Express?
- How can I recreate ratiometric data acquired in FACSDiva?
- How do I use R Integration with FCS Express?
- How does FCS Express implement software compensation?
- If my data does not have a Time parameter, can I create one?
- What is compensation?
- What is the compensation workflow in FCS Express?
- When acquiring spectral data, should my single-stained controls be "as bright or brighter" than my fully-stained sample?
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- Can a set of quadrants be both percentile and floating?
- Can I customize the display of my data from different instruments?
- Can I disable the live updating feature?
- How can I display all of my detectors for my Cytek data?
- How can I set FCS Express so my FCS 3.0 biexponential data looks the same as it did in the BD FACSDiva software?
- How do I display Summit data in FCS Express as it appears in the Summit Software?
- How do I fix the biexponential axes on a plot?
- How do I rescale CytoFLEX data so it displays as it did at acquisition?
- How do I update my density and contour plots created in Version 4 to use the newest color palette?
- What are resolution options?
- What is Biexponential and Hyperlog Scaling?
- What is the best way to set FCS Express to display FCS 3.0 data from FACSDiva on a 4 decade log scale?
- Where can I get more information regarding DNA analysis using the Multicycle AV?
- Why can’t I change my plot axis labels from the Name keyword to the Stain keyword?
- Why do my dot plots appear sparse and blocky?
- Why is the text on the right most label cut off my plot?
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- Can I create an output file that contains the same plot from each data file on a single page?
- How can I export my spectral data files from FCS Express with unmixing applied?
- How can I successfully export a GatingML file?
- How do the batch processing run modes differ, and why would I use them?
- Why do I get an “Old format or invalid type library” error when using Microsoft excel during batch analysis?
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- How are statistics in FCS Express calculated compared to how they are calculated in BD FACSDiva?
- How can I display my statistical data in Scientific Notation?
- How do I calculate EC/IC Anything?
- What is “Stain Index” and how do I calculate it with FCS Express?
- What is MFI (Mean or Median Fluorescence Intensity) and how do I calculate it in FCS Express?
- Why have percentages reported by quadrants changed after updating to FCS Express version 7.20.20?
- Why is the Geometric Mean being reported as NaN or ##ERROR##?
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Image Cytometry
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- How do I adjust the axes to display small particle data from Amnis CellStream?
- How do I choose which images and parameters to view in a Data Grid?
- How do I export/save data from IDEAS software and load it in FCS Express?
- How do I make my images in the data grid larger?
- How do I pseudo-color images in a data grid?
- How do I work with Amnis derived image cytometry data in FCS Express?
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- Can I display heat maps with my Image Cytometry data?
- Can I work with data from PerkinElmer Instruments?
- Do you offer 21 CFR Part 11 compliance options for the Image Cytometry Version?
- Do you offer image segmentation or image analysis?
- How can Attune™ CytPix data sets with images (.ACS files) be merged for high dimensional analysis?
- How do I use CellProfiler Data with FCS Express?
- How do I use ImageJ with FCS Express?
- What file formats are compatible with FCS Express Image Cytometry?
- Where can I find Nexcelom Resources and Applications?
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FCS Express on Mac
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Upgrading FCS Express
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- Can different versions of FCS Express exist on the same computer?
- How can I view and convert my V3 layouts to FCS Express 7?
- How do I import my version 3 security databases into newer versions of FCS Express?
- How do I update Density Plots created in Version 4?
- Is there an upgrade discount from earlier versions of FCS Express?
- Version 4 Internet Dongle Retirement
- Why are my density plots from V3 not displayed correctly in later versions?
- Why are there fewer outlier dots on my FCS Express 5 and later density plots than in V4?
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Clinical & Validation Ready
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- Can I get more information regarding the Add-Ons that can be purchased with a license?
- Can I lock my template based on an electronic signature?
- Does FCS Express have any features to help meet 21 CFR Part 11 compliance?
- What is the difference between the different types of Users that are available with a Security and Logging license?
- What is the difference between the Logging option and System Level Audit Trails?
