To merge Attune™ CytPix ACS files as ICE file format:

1. Click on blue plus button (+) in the Data List and choose Thermo Cytpix ACS Files (*.acs) from the File Type drop-down (example below).

2. Select the desired Attune™ CytPix data files and Click “Open file”.

*Note:  Steps 1 and 2 can be ignored if data files are already loaded for analysis or if it is desirable to load data through Save/Merge From Files dialog window.

3. On the Data (1) tab select Save/Merge from Files (2) to open the dialog window (3).

4. Under Select data files click on the Add button (4).

5.  Select Add using Standard Open Data Dialog (opens the dialog shown above) or Add using Advanced Open Data Dialog (shown below) that allows for the user to select data files from the Data List by activating the Data List tab (5).

*Note: If individual transformed .acs files to .ice files were created those files can be added.

6.  Select the desired data files and click on OK.

7.  Under Save Information select Save as Merged Files (6).

8.  Select the folder at the end of the Output File Name field (7) to determine the location to save the files, the file name, and modify the Save as type: to ICE Format Files (*.ice) (highlighted with red box) then click Save.

9.  Confirm Create File Identifier column has been selected (8).

*Note:  This will allow the user to separate individual data files contained in the merged data file.

10.  Choose the best export option for compensation under Save Options (9) and then click on Merge (10).

*Note: For some algorithms (like FlowAI), it is best to work with raw uncompensated data. In that case, it will be easier to run the algorithm before the merge.

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1. Click on blue plus button (+) in the Data List and choose ICE Format Files (*.ice) from the File Type drop-down (example below).

*Note: There will be two folders created when creating a merged .ice data file. One for Binary data and the other for Image data.

2. Select the desired merged data file and Click “Open file” and the data file will be added to the Data List.

*Note: The merged .ice data file can be used in the current analysis and gates that have been created will be applied.

3.  Click on the Tools tab (1) and select Transformations (2) to open the Transformations dialog window (3).

4.  Use the arrow next to the blue plus icon to select the desired standalone transformation or pipeline (4)(shown below).

5.  After the pipeline has been added, select the pipeline steps to be added by using the arrow next to the second blue plus icon (5).

6.  The Gate (6) can be modified by choosing a gate from the dropdown.

7.  The active file on the layout will be automatically used as the Template File (7).

*Note: By clicking on the ellipsis button you can Select Template From File or Select Template From Selected Plot.

8.  Select all parameters in the initial pipeline set up so that they are available for additional pipeline steps (8).

Additional information is available for transformations and pipelines. We are proud to offer world class support for FCS Express, please feel free to reach out with any questions.

To save the individual Attune™ CytPix ACS files as ICE file format.

1. Click on blue plus button (+) in the Data List and choose Thermo Cytpix ACS Files (*.acs) from the File Type drop-down (example below).

2. Select the desired Attune™ CytPix data files and Click “Open file”.

Note:  Steps 1 and 2 can be ignored if data files are already loaded for analysis or if it is desirable to load data through Save/Merge From Files dialog window.

3. On the Data (1) tab select Save/Merge from Files (2) to open the dialog window (3).

4. Under Select data files click on the Add button (4).

5.  Select Add using Standard Open Data Dialog (opens the dialog shown above) or Add using Advanced Open Data Dialog (shown below) that allows for the user to select data files from the Data List by activating the Data List tab (5).

6.  Select the desired data files and then click on OK.

7.  Under Save Information select Save as individual Files (6).

8.  Use the dropdown menu to select ICE Format Files (*.ice) next to Output File Format (7).

9.  Select the folder at the end of the Output Directory field to determine the location to save the files to (8).

10.  In the Output File Name the asterisk maintains the original file name. Users can add information or modify the file name entirely (9).

11.  Choose the best export option for compensation under Save Options (10) and then click on Save (11).