How do I export .ICE files from Thermo Cellomics HCS Studio?
Working with data from HCS Studio in FCS Express
De Novo Software and Thermo Scientific have collaborated to make the import of results and images from Cellomics high content systems possible. FCS Express is directly compatible with data exported from Thermo Scientific HCS Studio™ 2.0 Cell Analysis Software through the .ICE file format. The collaboration is an ongoing process and capabilities are continuously growing. For examples please see our applications below and our white paper highlighting the use of FCS Express for analysis of Fucci Cell Cycle data acquired on the HCS Studio 2.0 platform.
HCS Studio allows users to quickly acquire imaging data for a variety of standard assays. Your multi parameter imaging data can now be easily exported to the .ICE (Image Cytometry Experiment) format and analyzed in FCS Express or expanded upon using all of the tools and features FCS Express has to offer. See the Export Instructions below to learn how easy it is to export your .ICE files for use in FCS Express.
1. Choose Data/Image Export Tool from SW Utilities category.
2. Select the scan to export and click Export Configuration.
3. Choose .ICE (Image Cytometry Experiment) from the Export Format drop down.
4. Check the box for Include Images.
5. Choose the file type to export. (De Novo Software recommends .PNG to lower saved file sizes).
6. Click on the Browse icon to select an Export Location on your hard drive.
7. Click on Cell Feature Selection >>.
8. Click on the double >> Icon (A below) to add all Available Features to the SelectedFeatures.
9. Click on the Export icon (B below).
The .ICE format data will now be exported to the location you choose in step 6 and is ready to load into FCS Express.
Application Examples
By using the Thermo Scientific™ ArrayScan™ XTI High Content Analysis (HCA) Reader and Premo FUCCI cell cycle sensor, compounds affecting the cell cycle can be readily analyzed and reported with FCS Express.See for yourself by downloading the white paper or by using the free FCS Express Reader: Fucci Analysis (345 mB).
The Compartmental Analysis BioApplication in HCS Studio 2.0 was used to discern between tumor and normal tissue. FCS Express was used to further assess and visualize the data sets. See for yourself by downloading the PowerPoint Export or by using the free FCS Express Reader: Tumor Tissue Analysis.
tags: image cytometry
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Installation
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Licenses
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- Can I get more information regarding the Add-Ons that can be purchased with a license?
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- Can I convert my Cytek license from the countercode licensing option to another licensing option?
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Layouts & Loading Data
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- Are Beckman Coulter LMD files unique?
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- How do I change the display in my plots from one data file to another data file?
- How do I export .ICE files from Thermo Cellomics HCS Studio?
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- How are existing quadrants handled when an old layout is opened in version 7.20 and later?
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- Quadrants in FCS Express versions 7.20 / 7.24 and later
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Data Analysis
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- Caveats when using Biexponential Scaling with automatic Below Zero parameter detection in the presence of outliers.
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- Can FCS Express integrate Python scripts?
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- When acquiring spectral data, should my single-stained controls be "as bright or brighter" than my fully-stained sample?
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- Can a set of quadrants be both percentile and floating?
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- How can I set FCS Express so my FCS 3.0 biexponential data looks the same as it did in the BD FACSDiva software?
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- Can I create an output file that contains the same plot from each data file on a single page?
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- How are statistics in FCS Express calculated compared to how they are calculated in BD FACSDiva?
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- What is MFI (Mean or Median Fluorescence Intensity) and how do I calculate it in FCS Express?
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Image Cytometry
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- How do I adjust the axes to display small particle data from Amnis CellStream?
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- Can I display heat maps with my Image Cytometry data?
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- Do you offer 21 CFR Part 11 compliance options for the Image Cytometry Version?
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- How can Attune™ CytPix data sets with images (.ACS files) be merged for high dimensional analysis?
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- What file formats are compatible with FCS Express Image Cytometry?
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FCS Express on Mac
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Upgrading FCS Express
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- Can different versions of FCS Express exist on the same computer?
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- Version 4 Internet Dongle Retirement
- Why are my density plots from V3 not displayed correctly in later versions?
- Why are there fewer outlier dots on my FCS Express 5 and later density plots than in V4?
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Clinical & Validation Ready
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- Can I get more information regarding the Add-Ons that can be purchased with a license?
- Can I lock my template based on an electronic signature?
- Does FCS Express have any features to help meet 21 CFR Part 11 compliance?
- What is the difference between the different types of Users that are available with a Security and Logging license?
- What is the difference between the Logging option and System Level Audit Trails?
