Where can I find Nexcelom Resources and Applications?
Nexcelom Resources and Applications
The Nexcelom Cellometer Vision CBA Analysis System allows the user to quickly acquire imaging data for a variety of standard assays. Your multi parameter imaging data can now be easily analyzed in FCS Express and expanded upon using all of the tools and features FCS Express has to offer.
Additionally FCS Express is compatible with the Celigo plate based analysis system so you can easily visualize and report your plate based screens using the high content features in FCS Express.
Please see the resources below if you need helping claiming an FCS Express license from your Nexcelom purchase, if you would like to download standard analysis layouts, or if you would like to view some common application examples.
Every purchase of the CBA Vision instrument comes with 2 licenses of FCS Express. A countercode license that is locked to the vision instrument PC and a portable dongle license that you may use on any PC. To get started with your licenses you must first claim and configure them to your own user account. Detailed instructions for the claiming and configuration process are available in our Customer Claim and Configuration Instructions.
Click the links below to download some of the standard assay layouts for the Nexcelom Cellometer Vision CBA Analysis System. If you do not already have FCS Express installed on your computer, please download and install the free FCS Express Reader before clicking on the links.
| Application | Layout Download Link |
|---|---|
| Apoptosis/Necrosis | Download Apoptosis/Necrosis Layout |
| Cell Cycle | Download Cell Cycle Layout |
| Fluorescent Protein Expression | Download Fluorescent Protein Expression Layout |
| Fluorescent Protein Viability | Download Fluorescent Protein Viability Layout |
| Surface Markers | Download Surface Markers Layout |
(Note: If you have a purchased copy of FCS Express, you can add your .NXDAT files to the Data List in any of the layouts above in order to view your own data)
Application Examples
Assay Information:
Cellometer Vision was developed to count total cells and identify FITC-annexin-V positive apoptotic cells based on the microscopic and fluorescence characteristics of the cell sample. Detection typically takes less than 60 seconds and only requires 20µl of cell sample. Cell images and all analysis data, including cell size distribution histograms, can be saved for documentation. Data can also be easily exported to FCS Express Image Cytometry for further analysis.
Download and view the layout using the free FCS Express Reader: Nexcelom Apoptosis Assay.
Assay Information:
Cellometer Vision addresses the need for simple, rapid fluorescence cell counting. By capturing both brightfield and fluorescent images from the same cell sample, Vision can determine the total cell concentration and analyze how many of those cells are expressing GFP. Therefore, using only 20µl of cell sample, researchers can now rapidly identify fluorescence positive cells from a sample, analyze individual cell fluorescence intensity, calculate cell concentration, size and determine the GFP transfection rate in minutes on the lab bench.
Download and view the layout using the free FCS Express Reader: Nexcelom Fluorescent Protein Expression.
Assay Information:
Cell viability is routinely tested in cell culture laboratories and used in many assays such as cell proliferation, cytotoxicity, and tumor killing activity. Reliable and accurate viability testing can also be done using propidium iodide (PI), a fluorescent dye. Cellometer Vision was developed to automatically count total cells and propidium iodide fluorescence labeled dead/dying cells for fast, simple PI viability determination.
Download and view the layout using the free FCS Express Reader: Nexcelom Fluorescent Protein Viability Assay.
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Installation
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Licenses
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- Can I get more information regarding the Add-Ons that can be purchased with a license?
- Can I lock my template based on an electronic signature?
- Does FCS Express have any features to help meet 21 CFR Part 11 compliance?
- Does FCS Express have Quality Control features?
- Does FCS Express offer Single Sign On capability?
- How do I configure SQL Server to host a database for FCS Express?
- What database options are available when I purchase the Security option?
- What is the difference between the different types of Users that are available with a Security and Logging license?
- What is the difference between the Logging option and System Level Audit Trails?
- What SQL Server permissions are needed?
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- Can I share my USB dongle or countercode license with another user?
- Can I track usage of the internet dongle?
- Can I try out the Internet Dongle before I make a purchase?
- Can the administrator log users out?
- Do you have to be connected to the internet at all times with the Internet dongle?
- How can users be added to an internet dongle license?
- How do I activate my dongle?
- How do I change my internet dongle/site license password?
- How many people can be logged in at the same time?
- How many user accounts can I create?
- If a user left the computer running can the user log themselves out from another computer?
- What are the differences between the internet dongle and network licensing options?
- What happens if I lose my internet connection?
- What happens if the user leaves the computer without logging out?
- What happens to the users login in case of an unexpected interruption? For instance, a software crash, power failure, etc.
- Why am I receiving a message that FCS Express cannot connect to De Novo Software servers?
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- Can I convert my Cytek license from the countercode licensing option to another licensing option?
- How can I claim my license purchased through BD Accuri Cytometers?
- How can I claim my license purchased through BD Biosciences?
- How can I claim my license purchased through Nexcelom Biosciences?
- How can I claim my license purchased through Sysmex-Partec GmbH?
- How can I claim the FCS Express license that came with my Cytek instrument purchase?
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Data Analysis
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- Can FCS Express integrate Python scripts?
- Can I use the FlowAI script in FCS Express?
- Can I use the FlowClean R Script with FCS Express?
- How can I recreate ratiometric data acquired in FACSDiva?
- How do I use R Integration with FCS Express?
- How does FCS Express implement software compensation?
- If my data does not have a Time parameter, can I create one?
- What is compensation?
- What is the compensation workflow in FCS Express?
- When acquiring spectral data, should my single-stained controls be "as bright or brighter" than my fully-stained sample?
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- Can a set of quadrants be both percentile and floating?
- Can I customize the display of my data from different instruments?
- Can I disable the live updating feature?
- How can I display all of my detectors for my Cytek data?
- How can I set FCS Express so my FCS 3.0 biexponential data looks the same as it did in the BD FACSDiva software?
- How do I display Summit data in FCS Express as it appears in the Summit Software?
- How do I fix the biexponential axes on a plot?
- How do I rescale CytoFLEX data so it displays as it did at acquisition?
- How do I update my density and contour plots created in Version 4 to use the newest color palette?
- What are resolution options?
- What is Biexponential and Hyperlog Scaling?
- What is the best way to set FCS Express to display FCS 3.0 data from FACSDiva on a 4 decade log scale?
- Where can I get more information regarding DNA analysis using the Multicycle AV?
- Why can’t I change my plot axis labels from the Name keyword to the Stain keyword?
- Why do my dot plots appear sparse and blocky?
- Why is the text on the right most label cut off my plot?
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- How are statistics in FCS Express calculated compared to how they are calculated in BD FACSDiva?
- How can I display my statistical data in Scientific Notation?
- How do I calculate EC/IC Anything?
- What is “Stain Index” and how do I calculate it with FCS Express?
- What is MFI (Mean or Median Fluorescence Intensity) and how do I calculate it in FCS Express?
- Why have percentages reported by quadrants changed after updating to FCS Express version 7.20.20?
- Why is the Geometric Mean being reported as NaN or ##ERROR##?
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- Are Beckman Coulter LMD files unique?
- Can I find a support resource page for the analysis of Cytek data in FCS Express?
- How can I easily create the "filename" column in the "ExtraKeywordsTable.csv" file?
- How can I load data from the BD Accuri C6 Flow Cytometer?
- How can I load MACSQuantify files that were exported from MACSQuantify software version 3.0.1?
- How do I change the display in my plots from one data file to another data file?
- How do I export .ICE files from Thermo Cellomics HCS Studio?
- How do I tell FCS Express what plate size to use if that information is not included in the data file?
- How do I upload files to the De Novo Software FTP site?
- How do I use BD Accuri CFlow files with Multicycle DNA analysis in FCS Express?
- How do I work with images from the Thermo Scientific Attune CytPix?
- What is the Elapsed Time setting in the Gallios software and how do I convert it to real time?
- Why are there sometimes access violations when I save and load files?
- Why do I get the message that a data file exported from a FACSDiva™ Experiment is invalid?
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Image Cytometry
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- How do I adjust the axes to display small particle data from Amnis CellStream?
- How do I choose which images and parameters to view in a Data Grid?
- How do I export/save data from IDEAS software and load it in FCS Express?
- How do I make my images in the data grid larger?
- How do I pseudo-color images in a data grid?
- How do I work with Amnis derived image cytometry data in FCS Express?
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- Can I display heat maps with my Image Cytometry data?
- Can I work with data from PerkinElmer Instruments?
- Do you offer 21 CFR Part 11 compliance options for the Image Cytometry Version?
- Do you offer image segmentation or image analysis?
- How do I use CellProfiler Data with FCS Express?
- How do I use ImageJ with FCS Express?
- What file formats are compatible with FCS Express Image Cytometry?
- Where can I find Nexcelom Resources and Applications?
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FCS Express on Mac
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Upgrading FCS Express
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- Can different versions of FCS Express exist on the same computer?
- How are existing quadrants handled when an old layout is opened in version 7.20 and later?
- How can I view and convert my V3 layouts to FCS Express 7?
- How do I import my version 3 security databases into newer versions of FCS Express?
- How do I update Density Plots created in Version 4?
- Is there an upgrade discount from earlier versions of FCS Express?
- Version 4 Internet Dongle Retirement
- Why are my density plots from V3 not displayed correctly in later versions?
- Why are there fewer outlier dots on my FCS Express 5 and later density plots than in V4?
