FCS Express Knowledge Base

Why am I seeing a warning message when loading my Cytek data onto a layout object?

There is a user option in the Cytek Instrument Specific Settings called Cytek File Merge Rule shown in the screenshot below.

Previously, the default behavior was to only load the selected file (Raw or Unmixed).
Now (in 7.24.0024 and later) the default behavior is to automatically load both the raw and unmixed data when any Cytek file is loaded.
- This is helpful for several reasons:
  - An unmixed file can be viewed on a Spectrum plot.
  - The user will have access to the raw parameters when analyzing an unmixed data file.

If the data files are removed from the original folder structure that was exported from the Cytek instrument, then FCS Express cannot find the Raw or Unmixed counterpart.
- When FCS Express cannot find the counterpart file, it shows the warning message and just loads the file(s) that you chose to load in.

To only load the selected data file:

To allow FCS Express to find the Raw or Unmixed counterpart:

If you want the Raw parameters to get loaded automatically, the data files must be in the expected arrangement (an example is shown below).

- There is a folder called Raw and a folder called Unmixed.
- Those two files are within the same parent folder.
- The FCS files within the raw folders has to have the same exact name as the unmixed counterpart inside the Unmixed folder.
- If the raw and unmixed FCS files are divided into subfolders, the folder inside the Raw folder has to have the same name as the folder in the Unmixed folder.