Image Format Compatibility

De Novo software has collaborated with many imaging vendors to establish import compatibility for FCS Express.

FCS Express imports images, image analysis results, and image segmentation information from various instruments and image analysis packages to analyze data, create visualizations, and produce customized reports. While FCS Express is compatible with many formats, if there is no current compatibility for your image analysis results, you can likely work through an intermediate to generate files compatible with FCS Express.

FCS Express is compatible with the Image Cytometry Experiment data format (ICE Format), which is open to any producer of image cytometry instrumentation or software to standardize image cytometry data for import into other platforms. Please read more about the Image Cytometry Experiment Format, and view specifications on the ISAC Data Standards webpage.

In some cases, import compatibility with a single image analysis packages enables compatibility with many image formats (e.g. CellProfiler using Bio-Formats). In other cases, direct import of analysis results or links to imaging databases have been developed with specific vendors.

Compatibility Table

Product Name File Format
Amnis - IDEAS Version 6.2 and previous *.daf or *.fcs
Broad Institute - CellProfiler *.cpout
ChemoMetic - NucleoCounter NC-3000 *.ACS
Countstar *.ICE
Life Technologies - Tali Life Technologies Tali Data Folder
Molecular Devices - Image Express Velos (Isocyte) *.iso
Nexcelom - CBA Vision, K2, and Celigo *.NXDAT or *.ICE
ImageJ *.ijout
Perkin Elmer Vectra / InForm Software* *.tif or * .txt
Phase Holographic Imaging -  HoloMonitor App Suite *.ICE
Thermo Scientific - HCS Studio 2.0 *.ICE
Thermo Scientific - Attune CytPix *.ACS or .fcs
TissueGnostics *.ICE
Yokogawa - CQ1

*Note: Image to gate linking for Vectra InForm data sets is only currently available for exports from InForm software releases prior to InForm 2.4.1. Raw data import an analysis is compatible with all InForm software releases. Future compatibility with image to gate linking for all InForm exports is planned.  

Perkin Elmer Acapella folder is available in FCS Express 7 but is only rated for legacy data file formats. Please contacts with compatibility questions. Columbus database link was deprecated with FCS Express 7.  

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Manual and Tutorials

Image Cytometry in the FCS Express Manual

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