In a Column Table (one grouping variable), each column defines a group. These groups are defined by one scheme, perhaps "control vs. treated." You can have more than two groups in a one-way table, for example "placebo vs. low-dose vs. high-dose". In GraphPad Prism this table can be used to perform several types of analysis (e.g. Unpaired t test; Paired t test; Ratio-paired t test; Mann-Whitney test; Wilcoxon test; Kolmogorov-Smirnov test; Ordinary one-way ANOVA; and many others...).

• More details about Column Tables can be found in the GraphPad Prism User Manual.
• More details on how to set up a direct export from FCS Express to GraphPad Prism using Column Tables, can be found in the FCS Express User Manual.

In FCS Express, a user-friendly interface allows to organize data at your convenience by defining Row Titles and Column Titles.

In the example below, the "Gate 1 (# of Events)" statistic has been exported into a GraphPad Prism Column Table.

Once the Data Table is populated, creating graphs will take no time:

In an XY Table, every point is defined by both an X and a Y value. In GraphPad Prism this table can be used to perform several types of analyses (i.e. Nonlinear regression (curve fit); Linear regression; Fit Spline/LOWESS; Smooth, Differentiate or Integrate Curve; Area Under Curve; Deming (Model II) Linear regression; Correlation matrix; Correlation XY; Interpolate a Standard Curve).

• More details on XY Tables can be found in the GraphPad Prism User Manual.
• More details on how to set up a direct export from FCS Express to GraphPad Prism using XY Tables, can be found in the FCS Express User Manual.

In FCS Express, a user-friendly interface allows you to organize data at your convenience by defining X Values and Raw Titles, Y Column Titles and Number of Replicates.

In the example below, the "Gate 1 (# of Events)" statistic has been exported into a GraphPad Prism XY Table. The number of replicates is customizable and in this example has been set to 2 to allow sub-columns.

Once the Data Table is populated, creating graphs will take no time:

In a Grouped Table (two grouping variables) your data can be categorized by two variables. The idea of grouped variables is best understood by example. One grouping variable might be "male vs. female"; the other grouping variable is "control vs. treated." Every data point you collect is categorized by both grouping variables: "male vs. female," AND "control vs. treated". In GraphPad Prism this table can be used to perform several types of analysis (e.g. Two-way ANOVA (and mixed model); Three-way ANOVA (and mixed model); and others...).

• More details on Grouped Tables can be found in the GraphPad Prism User Manual.
• More details on how to set up a direct export from FCS Express to GraphPad Prism using Grouped Tables, can be found in the FCS Express User Manual.

In FCS Express, a user-friendly interface allows you to organize data at your convenience by defining Row Titles, Column Titles and Number of Replicates.

In the example below, the "Gate 1 (# of Events)" statistics has been exported into a GraphPad Prism Grouped Table.

Once the Data Table is populated, creating graphs will take no time:

If the keywords you wish to use for grouping are not already written into the data files, you can create an Extra Keywords file to access them in FCS Express. Please visit this page of the manual to learn more about adding Extra Keywords in FCS Express.

A template for easily creating an Extra Keywords file can be found in this Knowledge Base article.