High Content Screening

High Content Tools for Flow and Image Cytometry Data

FCS Express continues to lead the field by providing cutting edge high content visualization tools. FCS Express supports heat map plots for displaying plate based data, index sorting, high content screening, high content analysis, for flow and image cytometry data analysis.

Please note that the High Content is not necessary to visualize high dimensional data reductions/pipeline outputs.

Jump to HCS for Flow Cytometry Data

Jump to HCS for Image Cytometry Data

High Content For Flow Cytometry Data Analysis

HCS Compatible Instruments

FCS Express continues a legacy of innovation in the field by providing analysis capabilities for multiwell plate format flow cytometry data from the following instruments:

The imported data can be displayed on a per-well basis or as an entire plate in Heat Map format.

Index Sorting is also supported for:

Influx (BD Biosciences)
Summit Software (Beckman Coulter)
Diva (BD Biosciences)
Chorus (BD Biosciences)

Heat Maps for Flow Analysis

Heat maps allow the user to:
Plot any statistic on any parameter. Represent different files, statistics, or parameters simultaneously with multiple Heat Maps, Heat Map Overlays, or a Heat Map Radius (right).
Apply any kind of gate to Heat Maps, including well gates; wells can then be individually examined as any other type of plot that FCS Express supports. Work with High Content Screening or High Content Analysis data sets. Complete control over plate formatting and displays.

Heat Maps and Gating

Gates can easily be created on any heatmap through the Gating Tab (below-top row) or by right clicking on a plot and choosing Create Gate > Well (bottom-right). Gates created on a heat map can be applied to dot plots, histograms, etc., just like any other gate. The video below also shows heat map gating in more detail or the full webinar on working with heat maps in FCS Express may be viewed.

Color Schemes

Just like any other plot in FCS Express, Heat Maps are fully formattable: choose from several color schemes, and represent different files, statistics, or parameters simultaneously as Heat Map Overlays and/or Radius, as shown below. Click below to see a larger image.

Color Schemes

Display Any Parameter or Statistic

Displaying Additional Data, Parameters and Statistics

FCS Express supports multiple overlays from several samples that will appear as slices of a pie in the well. A Radius parameter may be selected that correlates the size of the well with the value of the parameter, for instance, setting the Radius parameter to the number of events gives a clear indication of how many cells are in a well but will also be coupled with heat mapping information for any other parameter. Overlays and radius parameters may be used in conjunction to get the most information from a single display.


Overlays (up to six)	Radius	Overlays + Radius

High Content for Image Cytometry Data Analysis

Heat Maps For Image Analysis

Data for heat maps can be generated from many manufacturers such as Cell Profiler, Nexcelom Celigo, Thermo ArrayScan, and more Image Data Format Compatibility is being added all the time.

Heat maps allow the user to:
Plot any statistic on any parameter. Represent different files, statistics, or parameters simultaneously with multiple Heat Maps, Heat Map Overlays, or a Heat Map Radius (right).
Apply any kind of gate to Heat Maps, including well gates; wells can then be individually examined as any other type of plot that FCS Express supports. Work with High Content Screening or High Content Analysis data sets. Complete control over plate formatting and displays.

Well Gates