To create an unmixing matrix in FCS Express simply use the Automatic Unmixing Setup wizard. The unmixing wizard functions similarly to the FCS Express compensation wizard making it easier than ever to move from standard flow cytometry data analysis to spectral data analysis. Select your single stain controls, parameters to unmix, and build histograms which will automatically set gates on positive an negative populations. Choose to calculate the matrix and get off and running with your analysis and reporting. The results will live-update whenever a change is made to the Unmixing setup.

With FCS Express you can quickly view your matrix and end members and apply it with ease to any data files associated with your experiment. Simply drag and drop your matrix to plots, apply it to all plots with a click, or have the matrix automatically be applied when loading data files in FCS Express. 

A Visualized Matrix can be created for any created or imported unmixing matrix.  FCS Express will automatically generate a Visualized Matrix by simply using a right-click and selecting Build Visualized Matrix on the desired unmixing matrix listed in the Compensation and Unmixing window.  Not only will you see a spreadsheet containing all the values for the detector intensity, a chart will display the Detector vs. Intensity for each single stained control.  FCS Express makes it easy and convenient to visualize your unmixed spectral signatures.