Application Examples
Watch all the videos within the first few days to get the most value from FCS Express!
IN THIS VIDEO:
In this webinar the teams at Spherotech and De Novo Software have collaborated to teach you about the beads and workflows you can use to perform daily QC checks on your cytometers, calibrate your data sets to MESF, and use FCS Express to do so in an mostly automated fashion without external Excel spreadsheets. The team from Spherotech discusses the technology and current workflows for using their Ultra and Rainbow Calibration Particles and the team from De Novo Software follows to teach researchers how to use FCS Express for quick reporting and tracking of QC results, utilize regression graphing that links directly to your data sets/gates, and automatic channel calibration from known MESF standards.
LEARN HOW TO:
- Prepare Particles and Set Acceptable Ranges
- Utilize Templates for Bead Calibration Assay Analysis
- Incorporate Integrated Spreadsheets and Charts for Reporting
 57 mins |
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Immunophenotyping
IN THIS VIDEO:
Join us as we analyze an immunophenotyping experiment in FCS Express. Watch as we insert and format plots,apply gates, markers and quadrants; add statistics to our layout; and, batch export our results to PowerPoint and Excel. We’ll then add more tubes to our experiment and analyze them side by side, using Panels. Rather than focusing on any particular procedure, we'll aim to quickly become familiar with many of the basic yet powerful features of FCS Express-- all in the context of a natural workflow for analyzing flow cytometry data.
LEARN HOW TO:
- Establish a Data Analysis Strategy
- Build a Gating Hierarchy and Insert Spreadsheets
- Use Batch Processing to Export Results
 49 mins |
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Index Sorting Data Analysis
IN THIS VIDEO:
Investigate how index sorting allows researchers to review the complete phenotype of every single event sorted into a plate and associate that event with all sorted and non sorted events. FCS Express 7 allows for analysis and reporting of index sorting data using Heat Maps at a click along with the standard plot offerings.
LEARN HOW TO:
- Insert a Plate Heat Map and View Index Sorted Data
- Create Gates Based on Wells of the Heat Map
- Format Heat Maps and Extract Relevant Statistics
 36 mins |
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Performing Antibody Titration
IN THIS VIDEO:
Learn why titration is an important step in the panel optimization process. Improperly titrated reagents can lead to increases in fluorescence background and poor overall resolution of your flow cytometry data. This webinar will help teach you the concepts that you can use in FCS Express to analyze your titration data. This webinar will also introduce the release of our Universal Antibody Titration Template that can be used for 5, 6, or 7 point titration experiments.
LEARN HOW TO:
- Add Titration Data to the Analysis Workspace
- Use the Universal FCS Express Titration Template
- Build a Titration Template
| 1 hour 9 mins |
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Stain Index Analysis
IN THIS VIDEO:
With FCS Express, learn how you can easily create your own Stain Index analysis and utilize your data set to obtain the best possible results.
LEARN HOW TO:
- Calculate Stain Index Using Integrated Spreadsheets
- Calculate Stain Index Using Custom Tokens
- Insert Regression Plot to Derive Statistics
| 48 mins |
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Dotmatics Biology Solution
IN THIS VIDEO:
The Dotmatics Biology Solution supports drug discovery across a wide range of biologic therapeutics including antibodies, CAR-T cells, and RNA therapeutics. The solution provides end-to-end biologics data management and analysis capabilities that fit into established complex workflows, while the platform enables structured data for users to easily access, collaborate, visualize, and analyze results.
 1 mins |
