Download Latest RUO Version

This page contains the final release notes for FCS Express 4.

To upgrade to the last release of FCS Express 4, please click here.

Release Notes

Version 4.07.0020 - July 16, 2014 (Research-Only)

Enhancements

  1. Added the ability to select macro-enabled Excel files in the file dialogs in Excel reports.

Bug Fixes

  1. Fixed a problem where outlier dots on density plots disappeared after switching to another page and back.
Version 4.07.0019 - May 2, 2014 (Research-Only)

Bug Fixes

  1. Fixes to handling of ICE data
  2. Stats from kinetics plots did not remember their gate information
  3. Resolved problems reading keywords from Beckman Coulter Gallios and Navios FCS files.
  4. Updated reading Building Blocks data
Version 4.07.0014 - December 5, 2013 (Research-Only)

Enhancements

  1. Added support for reading image data generated by Amnis IDEAS software - this feature is still in Beta. Please contact support@denovosoftware.com to enable it.

Bug Fixes

  1. Resolved problems reading keywords from Beckman Coulter Gallios and Navios FCS 3.0 files.
  2. Eliminated a crash that occasionally happened when re-calculating the automatic compensation, after dragging a gate from a GateView to change the compensation setup.
  3. Eliminated a crash that happened when changing certain histogram axes to the Lin/Log scale formula.
  4. Fixed a bug that prevented changing data files if a plot was displaying Building Blocks data.
  5. Eliminated a crash that sometimes occured when closing a layout, if there were other layouts still open.
Version 4.07.0011 - August 22, 2013 (Research and Clinical)

Enhancements

  1. Added support for reading plates from Guava instruments into heat maps.

Bug Fixes

  1. Eliminated a crash that happened when undoing a gate replacement, if the replacement was drawn on different axes than the original gate.
  2. Eliminated a crash that happened when opening a layout that had no pages.
  3. Updated export formats for specific clinical customers
Version 4.07.0005 - June 17, 2013 (Research and Clinical - all Enhancements and Fixes from the previous Research-only releases are included)

Enhancements

  1. Optimized loading image data to improve speed.

Bug Fixes

  1. Fixed a bug in the 5 Parameter Logistic (5PL) equation
  2. Fixed a problem with displaying ICE-format data, where cells outside a gate were not drawn correctly on the picture plots
  3. Updated export formats for specific clinical customers
Version 4.07.0003 - May 24, 2013 (Research-only release)

Bug Fixes

  1. Eliminated a crash that sometimes happened while moving polygon gates.
  2. Eliminated a crash from accessing the data source of tokens that were updated from data files, in the case where the layout had been saved unlinked.
  3. Eliminated a crash that could occur if FCS Express was in the middle of scrolling a page, and the computer or monitor went to sleep.
  4. Eliminated a crash that appeared in some cases while the compensation window was open and displaying a matrix, if the user clicked quickly on a different grouping of compensations in the list above.
Version 4.07.0001 - April 29, 2013 (Research-only release)

Bug Fixes

  1. Updated handling filenames of wells from data files inside BD Accuri C6 files to work with the latest versions of C6 software.
  2. Resolved a crash that happened if you created a linked gate from a gate with children, and unchecked the checkbox to Create linked child gates.
  3. Resolved a crash in batch processing which happened when you first saved a PDF file, then reran batch and appended to the same file.
  4. Fixed a bug in the 5-Parameter Logistics regression model.
  5. In previous versions, when doing a batch processing report where the target is an existing file, if the batch report was organized inside a folder in the batch window, the setting of the checkbox for "Ask for confirmation" was ignored.
  6. Improved error handling for the Cell Profiler file loader when it encounters unknown file types.
  7. Added more error checking on temporary files created when generated batch processing reports.
  8. Previously, when parameter math was set to run on loading new files, then if plots were created for new files by where dragging files from the Data list (or from Windows Explorer), parameter math was not run.
  9. Improved diagnostic information on moving gates.
  10. Fixed a crash that could occur when changing axes to use Log with Negative scaling, if there were many events along the axis.
Version 4.06.0006 - March 8, 2013

Enhancements

  1. Molecular Devices MDCStore data is now available for all Image and Plus customers.
  2. Improved the appearance of density plots imported into FCS Express from C6 files.

Bug Fixes

  1. Resolved a problem introduced in 4.05.0005, which prevented certain pictures from being displayed correctly on layouts.
  2. Fixed a crash that occasionally happend when moving or resizing a gate, if the the X parameter on the plot was displayed in the Biex transformation, and the Y parameter was displayed in linear.
  3. Fixed a bug that prevented layouts saved with embedded Acapella data from being reloaded.
  4. Fixed a crash that occurred after deleting a compensation from the compensation window, then dragging and applying a different compensation to a plot that had previously been showing the deleted compensation.
  5. Fixed a crash in the Data List, which happened in some circumstances after loading a layout unlinked, if the data list was set to sort based on keywords.
  6. Fixed a crash that occurred if there were errors when running parameter math, if the layout was set to run parameter math on newly loaded data.
  7. In 4.05.0005, the Collaboration portal did not always start correctly in network licenses of FCS Express.
  8. Resolved a bug in exporting data, if you selected a number of cells to export greater than the number of cells in the data.
  9. Previously, after pasting a marker into a histogram, if the stats grid was open, then it did not get immediately updated with stats for the new marker.
  10. Previously, if a layout was set to run parameter math on newly loaded data, and it also had a panel applied, then if there were errors during parameter math the data would not be displayed in plots.
  11. Other stability improvements.
Version 4.05.0005 - January 15, 2013

Enhancements

  1. Added support for Perkin Elmer Acapella data.
  2. Added support for Molecular Devices MDCStore data.
  3. Added Export to LabKey Server Batch Processing report.
  4. Updated the dongle drivers for Windows 8.
  5. Added functionality for specific clinical customers.

Bug Fixes

  1. Fixed problem in parameter math that would cause it to stop if a division by 0 was encountered in one of the operations.
  2. Fixed a crash when replacing cell number gates.
  3. Previously, undo of performing parameter math was not causing the plots to be refreshed.
  4. Improved error messages in image parsers.
  5. Made the concentration calculator more robust for handling ETIM and BTIM keywords from the Canto Clinical software.
  6. Previously, settings for dialogs with Do Not Show Again checkboxes were not remembered.
  7. Fixed a problem with copying certain dlls to the local cache.
  8. Updated the Multicycle fitting engine to fix a crash when it was unable to find a G2 peak.
  9. Removed extra brackets from titles of picture plots showing data from Life Technologies Tali.
  10. Improved the appearance of resized pictures.
  11. Fixed a crash that occurred when changing the batch processing options, while the batch processing actions window was open but collapsed.
  12. Changed the default value for the Tali options to load background images.
  13. Fixed the not remembering the gate selected for performing parameter math.
  14. Fixed a crash that could occur when displaying image plots, especially while loading a layout.
  15. Removed surrounding spaces on the Current User token.
Version 4.03.0011 - October 29, 2012

Enhancements

  1. Added support for Life Technologies Tali data.
  2. Improved speed of drawing color dot plots showing colors for many gates.
  3. Improved speed of showing keyword values in the data list.

Bug Fixes

  1. Fixed a problem that manifested on several people's computers, where the gate and quad outlines, and statistics in text boxes on plots, would not update after the gate or quad move was complete, until the user clicked outside the plot.
  2. In 4.02.0015, the Page Visibility feature did not work unless you opened and closed the formatting of the page tab - this was fixed.
  3. Fixed a problem in using the keyword in plot titles.
  4. Eliminated a crash that occured on startup if someone had copied a file from Windows Explorer into the clipboard and then deleted the file.
  5. Updated the Tali data reader to allow a csv file name of the same name as the folder.
  6. Fixed a problem with the Tali data reader that caused all fields of view to be drawn on top of each other if the background image was not loaded.
  7. Fixed a crash that occured on startup if the scrollable area of the layout was set to a particular ratio of the total window size.
Version 4.02.0015 - September 8, 2012

Enhancements

  1. Updated Import Diva Facsdiva Add-on to better comply with Diva 7.
  2. If the setting for allowing Diva numeric file names is turned on, the Import Diva Add-on now allows those files to be used when importing the experiment.
  3. Added the ability to add fractional color levels for contour, density and heatmaps.
  4. Added a checkbox for forcing directory creation in batch processing actions.
  5. Improved speed of loading layouts and rendering plots.
  6. New batch processing report functionality for specific clinical customers.

Bug Fixes

  1. Resolved an issue introduced in 4.01.0026, which caused the data file names in plots to be expanded to their full UNC names - this meant that they didn't match what was in the Data List, and batch processing did not work as a result.
  2. Fixed a decimal separator problem when using the "," as the decimal separator, which affected custom tokens and custom datas.
  3. Improved alphabetical ordering when loading files into the data list, to match what is displayed in Windows Explorer.
  4. Fixed the fitting on Multicycle plots when the data was magnified.
  5. Fixed a crash that happened in some cases if you changed density plot color levels to a threshhold value that was too low.
  6. Previously, if the settings on a color dot plot were set to show new gates, but there were no gates on the layout yet, then if you created the first gate on a different plot, the original plot would not be redrawn with the new gate color.
  7. Fixed a problem with histogram subtraction in layouts imported from version 3.
  8. Improved error handling when loading picture plots with bad data.
  9. Corrected a crash that occured when formatting histograms with multiple overlays and markers, if during the format the person deleted an overlay or deleted a marker.
  10. Resolved a problem with expression handling inside formulas inside custom data cells, which occured when loading the layout or editing the cell, if the formula did not evaulate to a valid number.
Version 4.01.0029 - June 14, 2012

Enhancements

  1. Improved speed of displaying plots and changing pages.
  2. Improved speed of loading layouts.
  3. Added custom functionality for specific clinical customers.
Version 4.01.0027 - May 24, 2012

Enhancements

  1. Improved speed when loading layouts from network drives.
  2. Improved speed of deleting lists of custom tokens.
  3. Improved speed of opening the Layout History window for layouts with a lengthy history.

Bug Fixes

  1. Fixed a bug in batch processing which could cause the layout to freeze after completing the batch.
  2. Fixed a problem that caused certain old version 3 layouts to be very large when resaved in version 4.
Version 4.01.0026 - April 12, 2012

Enhancements

  1. Internet dongle enhancements.
Version 4.01.0025 - April 2, 2012

Enhancements

  1. Added support for the ImageJ data format.
  2. Added support for ICE format version 1.1.
  3. Improved stability and speed of internet dongle and site license servers.
  4. Improved the speed of dragging and dropping plots.
  5. Added new functions RemoveText and ReplaceText in custom tokens.

Bug Fixes

  1. Fixed a problem that prevented people with network licenses from adding new users.
  2. Fixed a problem with clinical launch files using layout map IDs.
  3. In cases where custom tokens contained other custom tokens, deleting them would sometimes cause the program to hang.
  4. Renaming custom tokens in some cases would cause a crash.
  5. Improved the handling of Z-order when copying a group of plots to the clipboard and pasting.
  6. Reenabled right-click->Delete on batch actions.
  7. Fixed a problem with saving the "below zero" value of a biexponential formula - previously, if the value was saved as 0 and the layout was saved, it was sometimes reset to 200.
  8. Resolved a crash that happened when deleting a linked gate.
  9. Reenabled deleting non-admin users in the Internet Dongle license.
  10. Improved the speed of setting up the Email to Notify in the security configuration window and fixed a crash that occured on invalid entries.
  11. Fixed a crash that happened in very large layouts when deleting a gate that was visible or used on many other plots.
  12. Improved the messages shown when building histograms for automatic compensation.
  13. Fixed undoing assigning compensations to a plot through dragging a compensation.
  14. Eliminated problems that happened when undoing signing a layout.
  15. Fixed a Windows resource problem that occured when using the navigator windows in very large layouts.
  16. Sped up typing in the Alerts windows.
  17. Fixed a crash that happened when double-clicking on a layout icon on the desktop, if that same layout was already open in FCS Express and was in the process of being closed.
  18. Allowed entering values lower than 90 for the JPG quality setting.
  19. Fixed a problem that caused files in the data list to be loaded in a non-alphabetical order.
  20. Prevented a crash when moving linked gates in a Gate View.
  21. Forced tokens to update to empty values when the data list is cleared, and the plots that the tokens are pointing to become empty.
  22. Prevented parameter math from running on embedded data files, when the option is set to run when files are loaded.
  23. Fixed the file open dialogs to properly display Windows folder shortcuts.
  24. Previously, outliers were not always drawn correctly for each outlier in contour plots where the palette is set to use logarithmic levels.
  25. Fixed a problem that caused Multicycle plots to perform an incorrect fit if the data was magnified.
  26. Resolved a memory leak that occured when copying plots to the clipboard.
Version 4.01.0018 - January 13, 2012

Enhancements

  1. Sped up plot display when adjusting manual compensation.
  2. Added copy protection information (countercode, dongle, internet dongle) to the About window.

Bug Fixes

  1. Fixed the problem that caused layouts saved or published from inside a batch processing report to lose plots on the 2nd pages.
  2. Fixed the duplication of keywords that occured on certain layouts after publishing a layout inside a batch process report.
  3. Fixed setting permissions on a published layout inside a batch processing report.
  4. Resolved a crash that could occur in the standalone license when the computer was waking up from being asleep.
  5. Restored the ability for network users to double-click on layouts and open them immediately.
  6. Fixed the display in the About window that caused standalone licenses to appear to be network.
  7. Resolved display problems in the automatic compensation setup messages.
  8. Fixed a crash that could occur when dragging and dropping a compensation matrix in a text box, if there was already other text in the text box.
Version 4.01.0015 - December 24, 2011

Known Limitations

  1. Network users will lose the ability to double-click on layout files, and automatically open them in FCS Express. Instead, you will need to first open the program, then open the layout. This will be resolved shortly in an upcoming release.
  2. If you are publishing the layout inside an XML in batch processing, then if there are hidden pages at the beginning of the layout, the plots on subsequent pages may not appear in the layout. This will be resolved shortly in an upcoming release.
  3. If you are using the security module with an ElevateDB database, the upgrade will not be backward-compatible. Please contact us for assistance.
  4. Internet dongle users are required to upgrade.

Enhancements

  1. Revamped and improved compensation infrastructure. There is a new dockable window with compensations, and an improved method of setting up automatic compensation.
  2. Improved management for the security system and internet dongle users, including the ability to log people out remotely, and to set up notifications for login failures or other events.
  3. Improved memory usage for picture plots.
  4. Improvements to the Proliferation fitting algorithm.
  5. Improvements to the contour plot outliers.
  6. Added options for gate text box placement.
  7. UI Enhancements: new buttons for zooming, and fewer clicks required to log out and change preferences.
  8. Implemented Ctrl + mouse wheel for zooming.
  9. Additions to the manual for Electronic Signatures
  10. Added options for changing the Edit/Selection colors of plots
  11. Improved speed of editing Users and Groups in the security module.
  12. Introduced a caching mechanism, to improve the speed of starting up FCS Express when running from a network drive.

Bug Fixes

  1. Fixed a problem that led to statistics not updating immediately if a plot had percentile quadrants, and the gate that the plot was gated on was modified, or the data or compnesation was modified, and the plot was not showing on screen.
  2. Resolved a crash that could happen if bad information was on the clipboard.
  3. Previously, dragging and dropping text boxes into plots did not work.
  4. Resolved crashes when importing certain CFlow files.
  5. Eliminated a crash that sometimes happened if the monitor turned off while FCS Express was running.
  6. Fixed a problem that led to a picture being specified as having 0 width in an XML export
  7. Fixed a potential hang after exporting to Excel.
  8. Fixed an occasional crash that happened when moving gates in plots with biexponential axes.
  9. Other stability improvements
Version 4.00.0057 - October 18, 2011

Enhancements

  1. Added the ability to read Nexcelom data files.
Version 4.00.0054 - July 18, 2011

Fixes

  1. Fixed a problem introduced in 4.00.0053 where the database repositories could not be installed.
  2. Fixed a problem where plots based on custom data using tokenized strings were not always updated when the layout was loaded.
Version 4.00.0053 - June 28, 2011

Enhancements

  1. Added the ability to read data in the ICE and ACS standard.
  2. Improved memory management of image data - loading image data sets is no longer limited to the amount of available RAM.
  3. Added SSMD and Z-Prime
  4. Now supports having more than one config file in the program settings folder - you will be prompted for which configuration to use.
  5. PDF version 1.6 is now supported in PDF batch reports.

Fixes

  1. Fixed a bug where quad stats did not immediately update visually when Pause between iterations was turned on in Batch Processing.
  2. Improved handling of scatter parameters from iCyt and InFlux data files so that they are displayed in linear by default.
  3. Renamed references to Isocyte from Molecular Devices to be ImageExpress Velos.
  4. Improved how the gate color precendence is calculated in dot plots.
  5. Previously, when adding an overlay to a histogram, the compensation was not automatically applied.
  6. Fixed reading in the parameter name keywords from the second data set of Beckman Coulter data files.
  7. Fixed a crash in drawing gates if the gate was too small.
  8. Fixed problems with Multicycle and CellProfiler data when used with Windows regional settings where the decimal separator is not '.'
  9. Enabled resizing columns in data grids.
  10. Enabled changing plot types for plots imported in version 3 layouts.
  11. Fixed a problem in exporting data files more than once.
  12. Uninstall now removes the program settings folder where possible.
  13. Improved the way range selection is calculated in Cell Number gates on data grids.
  14. Improved the appearance of options dialogs and wizards if Windows is set to use large fonts.
  15. Resolved a crash that happened when you started up a network version but did not have proper permissions on the network share.
  16. Previously, the % Positive statistic was not always recalculated on time.
  17. Improved the way the CFlow page options are handled during import.
  18. Ensured that histogram overlays from a CFlow import are all colored uniquely.
  19. Improved handling of dragging and dropping tokens onto plots.
  20. Fixed a crash that could happen if Office was not installed on Windows.
  21. Reduced flickering when navigating through tables inside text boxes.
  22. Fixed a bug that the parameter label settings were not always remembered in the axis label formatting category.
  23. The active state of batch processing actions is now remembered properly.
  24. Fixed the ability to magnify heat maps.
  25. Removed the program expiration date of Jan 1, 2012.
  26. Improved handling of Office .docx, .xlsx and .pptx files.
  27. Stopped requiring validation of batch actions that were deselected.
  28. Fixed a crash that occured if percentile quads were set to 100%.
  29. Fixed the legend color in bar plots.
  30. Improved the way axis labels are shown in surface plots.
  31. Other stability fixes.
Version 4.00.0023 - April 5, 2011

Enhancements

  1. Added several regression models: 5PL, 4PL, two-phase exponential fit, power series, Gaussian, Bolzmann, exponential association, sine wave, two-site binding, parabolic, cubic
  2. Added a node for plots with "No Data" in the Data Navigator.
  3. Enabled resizing columns in data grids.

Fixes

  1. Improved support for Multicycle in languages with a comma as the decimal separator.
  2. Changing parameter names in the header did not propagate changes to open plots.
  3. When dragging a data file into a histogram to create an overlay, an undo was not added to the undo system.
  4. Fixed a crash when you try to open the Axis formatting options on the ribbon for a bar chart.
  5. Improved stability when right-clicking in table cells and placeholders.
  6. Improved stability with Isocyte data when switching options between loading and not loading images.
  7. Fixed a crash that happened when closing a layout that had several dockable windows docked together.
  8. Previously, if a plot was using a parameter in a data file that no longer existed, it could trigger a crash.
  9. Fixed autosizing of statistics windows when loading version 3 layouts.
  10. Other fixes for loading layouts from version 3.
  11. Fixed a crash that could happen when displaying a log parameter with a non-automatic range.
  12. Fixed a crash when deleting an overlay that was used to normalize another one.
  13. Previously, dragging a file from the data list off and then back on to the list sometimes caused a crash.
  14. Fixed a problem when dragging a plot from one layout to another.
  15. Previously, FCS data files larger than 100 MB could not be written properly.
  16. Previously, if an Excel report specified a worksheet name which was blank or only had tokens in it, this could lead to an error.
  17. Fixed a problem with the Accuri import when trying to import histograms which had overlays, but not all overlays had data files.
  18. Fixed the page layout calculations in the Accuri import wizard.
  19. Other stability fixes.
Version 4.00.0010 - February 15, 2011

Enhancements

  1. Added the ability to take layouts saved in FCS Express 4 Plus and open them in FCS Express 4 Flow.
  2. Allowed Word batch processing export to use more than 60 merge fields.
  3. Added a warning dialog box when you save version 3 layouts.
  4. Updated the program icons to be distinct between Flow, Image and Plus.

Fixes

  1. Tokens for Multicycle statistics on a different page from the original plot did not always update in time.
  2. Multiselecting histogram overlays in the Overlays formatting category did not allow you to change the parameter.
  3. Previously, files without extensions were not being shown in the open data dialogs.
  4. Improved the way overlay lists are combined in the Quadrant and Overlays formatting categories when multiple plots are selected.
  5. In the Diva import, if a tube name had characters that were invalid for a filename (ie ;?/\) it was not displayed properly in the dialog that asks to select the acquisition filename.
  6. In the Diva import, if the files were located in a different folder than the XML file, then they were not found even if you selected them in a dialog.
  7. In the Accuri import, the axis options are now set to automatic by default.
  8. Fixed the background color property for picture plots.
  9. Fixed a crash that happened if you were in the middle of renaming a custom token and closed the layout.
  10. Fixed a crash in deleting multiple cells in a custom data grid.
  11. Fixed loading the background image for picture plots.
  12. Resolved a crash when displaying bar plots with a log axis.
  13. Fixed a problem with setting permissions for publishing using the advanced publisher.
  14. Fixed a crash that happened when the Select User dialog is open, and you double-click on a layout file in Windows Explorer.
  15. Prevented a crash in the Align feature when plots are grouped together.
  16. Fixed a problem changing the legend text in the Edit Preferences window.
  17. Prevented a crash when deleting position markers in Multicycle plots.
  18. Prevented a crash when you drag 2 or more files from Windows explorer into the Data List.
  19. Previously, changing the order of the batch actions was not always remembered.
  20. Improved the program behavior when you switch the number of monitors on your computer.
  21. Prevented a crash when you copy and paste a gate onto a plot from a plot showing different parameters.
  22. Stability fixes when loading in version 3 layouts.
  23. Other stability fixes.
Version 4.00.0000 - January 9, 2011
Initial release of FCS Express Version 4