FCS Express: Advanced Quadrants

FCS Express supports three different kinds of quadrants: Standard, Floating (non-rectangular), and Percentile. FCS Express also supports quadrant to gate linking.

  • Standard quadrants are the traditional crosshair dividing the plot into four rectangular sections. To change the size of the quadrants, click on and move the central vertex.
  • Floating quadrants provide flexibility for subsets that cannot be demarcated with Standard quads. With floating quadrants, all vertices (the center and one on each sidewall of the plot) are moved independently. Therefore, you are no longer restricted to rectangular shapes. In the second plot below is an example of Floating quads.
  • Percentile quadrants allow you to quantitatively determine the quad position, based on your data.  You decide the percentile along either or both axes where where the central vertex should fall, and FCS Express will take care of the rest. These quads will update accordingly as the data is changed.
  • Quadrant to gate linking allows for gating on a quadrant. You can easily convert a quadrant to a gate with a click of a button, and then apply or show the resulting gate on other plots, just as if it were a traditionally drawn gate.

Full statistical analysis can be performed on all types of quadrants.

Standard quadrants are unable to demarcate the subsets denoted by the different colors on the plot above. Floating quadrants better fit the populations and allow for the accurate calculation of statistics. Percentile quadrants (here set at 20% and 90% for the X- and Y-axis, respectively), enable quadrants to be set automatically and to update with your data.