All flow cytometry software packages include features for plotting, gating, and calculating basic statistics. When the team at De Novo Software observed researchers and scientists in both academic and commercial settings, it became obvious that the challenges they faced with data analysis were not in producing plots, gates, and statistics.
Instead, the real challenges were downstream activities of performing advanced calculations, advanced statistics, graphing, presenting, and reporting results.
If you are currently using FlowJo and are having trouble arriving at publication quality results, find it hard learning an unfamiliar software interface, find yourself constantly having to copy and paste tables and graphics to other software packages, then FCS Express might be just what you are looking for!
With FCS Express your analysis is your final result and is exportable in many formats at any resolution desired by the publisher.
FCS Express looks, feels, and works much like familiar Microsoft* Office software so with FCS Express, you are already almost an expert with the software before you even start.
In FCS Express spreadsheets and graphs are integrated and directly linked to your data and gates. Say goodbye to copy and paste!
Live updating, publication quality, final results are built into FCS Express 7
"FCS express is a much better program than my good old go to Flow Jo. The support is amazing, as typically taken care of immediately, and honestly 24 hours is the typical. Flow Jo has great webinars, but FCS Express has a bunch of webinars as well, and again cannot beat the technical support. So glad I was put into a position where I needed to leave my traditional go to [FlowJo], as FCS express has won me over. Still new so may still have questions but love it so far.”
Karen F. Underwood, MS
"The customer service, and the functionality of the software is by far the best I’ve seen. Unless forced to, I will never touch FlowJo again"
"FCS Express has revolutionized the way we think about data analysis. Not only are ALL the analysis tools available at your fingertips, the ability to work in a “Microsoft-like” environment makes the whole process intuitive and a great time savings. Our users have shared the same level of enthusiasm about our decision to switch to FCS Express for all our data analysis needs.”
"I am a big fan of FCS Express, it's the first tool I used to analyze flow data and I absolutely love it! Thank you for building that wonderful tool! It helped me get my Masters and also get 6 papers published”
"You guys have the best customer service of ANY product I own."
"We have been offering FCS Express to our shared resource lab users since 2017. Thanks to the excellent support provided by De Novo Software, we have had a steady increase in users adopting this platform. De Novo Software is ready with classes, phone and online support and fits in well with the research services environment. Our users particularly like the user friendly interface!"
"My function here at Labcorp is to set up a work flow process for over 350 cases a day companywide so without a doubt I am choosing FCS Express to accomplish this. In my opinion there just isn't another software option for us. I need a user friendly package that will accommodate our current complex multicolor analysis as well as be able to expand for future growth, provide high quality reports for our clients, offer a virtual component for those customers that want to offer tech only flow or more closely interact with their patient results and the big challenge is that this has to happen in an extremely high volume reference laboratory. De Novo Software has been able to provide us with the features that we need and their technical support is extraordinary. I can't imagine a successful clinical lab without FCS Express; it is the "go-to" Clinical package."
Horacio Vall CLS, SCYM(ASCP)
Vice-President Science & Technology, Doctor of Biochemistry Flow Cytometry Discipline Director
Laboratory Corporation of America Holdings
"Within a short period of time, we created a drastically improved analysis protocol in FCS Express and it seems that every day we discover new features, such as tokens, alerts, panels, etc. that easily allow us to improve the quality of the analysis. Above all, I am extremely impressed with the excellence of the support staff that is always available to answer questions and courteously and positively responds to suggestions. In my opinion, FCS Express is one of the best programs available today for clinical flow cytometry laboratories."
Raul Braylan
Former Medical Director, Flow Cytometry Laboratory
Shands Teaching Hospital at the University of Florida
"FCS Express is the most intuitive, flexible, and comprehensive flow cytometry software we have ever used. The company is extremely responsive and supportive with helping us to accomplish very complex analyses and make sure the software is meeting our clinical laboratory needs. We have gone through several instrument and LIS transitions over the past 10 years and have been able to rely on the software to help alleviate the pain of those changes. We would highly recommend this for any flow lab!"
Carrie Gifford
"Holly was exceptional in a myriad of ways. She quickly helped me resolve an issue with a software upgrade and with updating our config file from a recent license renewal. She treated our issue with the proper level of urgency and was clear with instructions to assist to resolve our challenges completely. I wish all interactions with a vendor were as smooth as the experience was with Holly. Thank you so much for the support."
"I am highly satisfied with the entire FCS support team, which is fast, uncomplicated and efficient in answering (sometimes also silly) questions and helping with solutions. In my opinion, this is one mega strength of your software product, which - as good and flexible it is, it also is demanding - and why I therefore unreluctantly recommend FCS Express to colleagues."
Andrea Harrer
SALK
"I think the customer service is great! Any time I'm in need of assistance I get a quick reply that solves the problem I am having."
Jennifer Murray
Lab Manager
CHOP
"My experience with DeNovo customer support is always great and quick. Holly is the best help!!"
Noelis M Pieto Avilés
Technical Supervisor-Flow Cytometry
Hospital of the University of Pennsylvania
"Katherine is quick to reply and her answers are thorough. She is all around amazing!"
Cora Chadick
Scientist III, Field Applications
Thermo Fisher Scientific
"De Novo support team is the best I have ever met! I sincerely appreciate Robin Kamucheka who always immediately answer my questions and even made video for me for some small questions. The support team give me big confidence to continue using FCS express!"
Huang Wang
Mater Research Institute
University of Queensland
"Juan Vazquez is very knowledgeable, and I received prompt FCS Express-related support."
Manoj Pandey
Researcher, CCHMC
"Juan was super quick to respond, asked great clarifying questions, and was very helpful at getting me the information I needed."
Lunden Simpson
Lab Manager
NCSU College of Veterinary Medicine
"Thanks so much for your help, Greg! You not only helped to address the main issue, you helped me learn a much easier way to analyze my larger data sets!"
Kimberly McKinney
Manager
Pediatric Oncology Research Lab
"I used to be in the FlowJo camp but I'm now totally converted to the FCS Express camp! Loving your software!"
"Amazing support from Katherine! She took the time to address all my questions and walked me through various solutions to the problems I was encountering. She was supportive and kind during the entire session."
Amanda Pacheco-Spiewak
PhD Candidate and Fellow
University of Maryland School of Medicine
"Robin was amazing and very knowledgeable to help me build the template we need for our new assay. I really appreciate her help."
Woan-Nu Yao
Senior Specialist, Quality Control
Lyell Immunopharma
"I was blown away that Greg not only replied quickly to my email, but he also set up a Teams meeting to address my issue. His solutions could prevent a lot of time being lost on data analysis in my lab. Thank you Greg!!"
Sean Sepulveda
Senior Research Associate
City of Hope

Copy and pasting from your data analysis software to Excel*, Prism‡, and other spreadsheet or graphing tools?
Have you ever created a chart in Excel and then needed to slightly move a gate resulting in an entire copy and paste of the flow cytometry data sets back to Excel?
Tweaking the formatting of flow cytometry graphs, plots, and axis to make them presentation ready?
Have you ever had to put a white text box over an axis label to simply present a graph?
Fighting with low resolution graphics just to achieve the requirements of your publisher?
Have you ever had to use multiple graphic editing tools just to arrive at a publication ready figure?
If you are transitioning from FlowJo to FCS Express make sure and check out our short video describing the similarities and differences between both software packages to help you get a head start with your new analysis in FCS Express. Our short video provides guidance on some differences including:
If you are transitioning from FlowJo to FCS Express make sure and check out our short video in continuation of our series, Transitioning to FCS Express, we explore Compensation similarities and differences to FlowJo.
Customer Testimonials
With other software packages you only get basic plots and statistics which requires you to copy and paste to external software packages to create a final result. In some cases you may be using multiple external tools to generate spreadsheets, graphs, PowerPoint* presentations, figures for publication, and R† scripts before you arrive at a final result.

With FCS Express analysis IS YOUR FINAL RESULT!
FCS Express utilizes integrated spreadsheets so your graphs and statistics update as your gates change. All graphics are directly exported to PowerPoint in high resolution and you can even choose to export individual graphics at up to 700 DPI for the ultimate ease in moving from analysis to publication ready result.

*PowerPoint and Excel are trademarks of Microsoft Corporation | †R is a trademark of the R Foundation | ‡Prism is a trademark of GraphPad Software, Inc. | FlowJo is a trademark of Flowjo, LLC