Feature Category: Validated Research

Standardize your flow cytometry data analysis

Maximize efficiency and improve quality with SOPs

Often, flow cytometry experiments and analyses are performed by different members of the same lab or at different sites, which can lead to inconsistencies in the way results are reported. Technical Standard Operating Procedures reduce variation between experiments, so why not use them for analysis as well? The Standard Operating Procedure tool in FCS Express helps ensure reproducible results every time by allowing you to implement a custom Standard Operating Procedure designed to your specifications that is accessed directly within the software while analysis is being performed.
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Consistency is critical

Standard Operating Procedures help your flow cytometry laboratory perform analysis the same way every time by following step-by-step instructions directly in FCS Express through an intuitive and easy to use interface.

Control every detail

First, build your SOP to define each necessary step. Then, when needed, add simple conditional statements to allow moving to the next step in the protocol. Finally, have users run the SOP during flow cytometry analysis to ensure consistency.
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Unify the team

SOPs allow labs to ensure uniformity and quality across the team and multiple sites while eliminating redundancies and errors.  Improve communication by ensuring all lab members are performing analysis the same way. 

Training made easy

Teaching FCS Express may be simplified by designing your own step-by-step instructions based on the needs, instrumentation, and experiments performed at your location to guide users through any analysis.

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Tokens Get You Results

Tokens are live updating text, statistics, and system information from your analysis

Making sure your analysis is annotated correctly and organizing your statistics is crucial for all scientists. FCS Express allows you to insert live-updating text called Tokens anywhere in your analysis. They can display plot statistics, file keywords, gate and layout properties, and even system information from your computer. Tokens will accelerate your time to results.
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Keep Track of Your Experiments

Tokens allow you to access the date and time that your files were acquired and other information that you entered at the time of acquisition. With tokens you will never need to enter information manually, or copy and paste, if you have entered it when you acquired your files. Never sift through your lab note book for important dates and experiment information again.

Incorporate Stats Directly in Your Plots

Tokens can be dynamically incorporated into plots to quickly summarize your analysis in place or to create stunning figures for publication and collaboration.

Tokens are as Easy as a Drag and Drop

Easily add tokens anywhere by dragging and dropping. Have a plot with a gate on it? Drag and Drop the gate to insert a token for MFI. Have a data file open? Drag and Drop to access keywords and show the Tube Number. Need statistics from your stats tables somewhere else? Drag and Drop to place the statistics anywhere in the layout.
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Customize and Create

Custom Tokens can help you create a custom value for any mathematical expression, allow you to perform conditional IF/THEN statements, convert numeric values into simple text such as Positive or Negative all while remaining live updating so as you move a gate, your custom values update immediately.

Tokens in the Clinical Setting

FCS Express IVD incorporates special tokens that are critical for Clinical labs. These tokens can be triggered by any numeric or text values and allow for real time Statistical Classifiers, Font Formatting, Page Visibility, and Batch Process Action triggering.

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Publication Quality Graphics

Eliminate the need to use multiple software packages to present your data

Generating high quality graphics and reports to communicate your results can be hard with other software packages and may involve using many different image editing tools. When you are ready to publish and present your data FCS Express has you covered by supporting direct high-resolution exports to PowerPoint, PDF, and six image formats at up to 750 DPI resolution. Never fight with low resolution graphics and bulky workarounds again when all you want are results!
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Skip the Copy and Paste

In FCS Express your analysis is your presentation, figure, or final report. Simply click to export directly to PDF, PowerPoint, or in the image format of your choice at the resolution you desire.

Full Reports at High Resolution

Your analysis can be set up as a full report including high resolution graphics. Your reports are linked up directly to your data so as you move a gate your report updates immediately. When you are ready to publish, simply export to the format of your choice.
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High Quality PowerPoint Presentations

]If you have ever struggled with low quality PowerPoint exports from your current software then FCS Express is for you. Your analysis and plots are exported from FCS Express at the highest resolution possible and individual plots and objects will arrive in PowerPoint also as individual objects so you can move, resize, and format all objects independently in PPT if needed.

Big Experiments? Big Data? No Problem

You never have to sacrifice the quality of your reports and figures due to the size of your experiments or number of data files with FCS Express. The easy to use batch processing feature in FCS Express allows you to export at the same quality across any number of samples, to multiple formats (PowerPoint, Word, Excel, PDF, Images) simultaneously, all with the click of a button.
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Visualize Your Data Easily and Automatically

FCS Express Supports Seven Data Scaling Methods and Autoscaling for Flow Cytometry Data Analysis

There are a wide variety of ways that flow and image cytometry data is displayed in todays challenging environment. Depending on the instrument, and the parameters, data can displayed in different ways (i.e. linear, log, biexponential). You need a tool that can accommodate all the different scaling options required by different instruments on the market.
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Automatic Axis Scaling

FCS Express supports all of the commonly used axis scalings for your flow cytometry data files. In most cases your data will appear exactly as it did during acquisition automatically so you can spend less time formatting plots and more time getting results.

Flexible Axis Scaling

When you need to change between different axis scalings simply choose from the FCS Express drop down which scale to apply to your data. All commonly used axis scalings for flow cytometry data analysis are available for use.

Instrument Specific Settings

Not all instruments are created equal with some data from instruments acquired with different maximum ranges. No matter what the dynamic range of your cytometer FCS Express has you covered with options to scale your plots automatically according to the cytometer type so all you have to do is analyze.
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Display Data According to Your Needs

Axis scaling is performed on a plot-by-plot basis meaning that you can compare different scalings side by side to make sure you are making the best decisions when identifying populations in flow cytometry data analysis. When you are ready to share your results simply use the plot that best tells the story of your data.

Biexponential for BD FACS Diva

As a result of our close collaboration with BD, data files opened in FCS Express will look exactly as they did in Diva and use the same scaling/bi-exponential transition points.

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Mac and PC Compatibility

Seamlessly move between computers and operating systems with your flow cytometry data at any time

In todays environment, users need the flexibility to use different computing tools. Even within a single lab, some researchers will use Mac and others will use PC. Even a single individual may use different computers at different times. Software needs to be able to accommodate these challenges. FCS Express runs the same way, with the same features, on Mac or PC or Both!
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Flexibility to Analyze Where You Need To

Flow cytometry data analysis is not always performed in the lab. FCS Express licenses are flexible and portable. Get analysis done when and where you need to. They can be used on any computers you would like, Mac or PC, or both!

Same Features, Different OS

It does not matter if you are performing day to day flow cytometry or running complex high dimensionality reduction algorithms. The same features will be available in the same place in FCS Express regardless of your operating system of choice.

Use on Unlimited Computers

Licenses of FCS Express are not “locked” to a PC or a Mac which means you can download, install, and use FCS Express on as many computers as you would like. With De Novo Software’s convenient Internet Dongle Licenses accessing the software only requires an internet connection.
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Move between computers and colleagues

Start a flow cytometry analysis on your PC in the lab and complete it on your Mac at home. Share your analysis completed on your Mac with your colleagues who use PC. With FCS Express you have the ultimate flexibility.

Use the Right Computer for the Job

The computer you use to perform simple flow cytometry analysis may not be the same computer you use for highly complex immunophenotyping, plate based screens or, tSNE/vSNE / SPADE / PCA calculations. Use FCS Express to move seamlessly between Mac and PC, high performance or everyday, and use the right machine for the job.

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