Feature Category: Validated Research

Plots with Flexible Formatting

FCS Express has the visualizations you need to move from flow cytometry analysis to publication

Communicating your results visually is an important aspect of any analysis, report, or publication. With other cytometry analysis packages researchers tend to rely heavily on external software tools to prepare their plots and figures for presentation and publication. With FCS Express every aspect of your plots can be easily customized directly in the software so you arrive at the visualization needed during analysis. Whether you are looking for a colorful backgated plot or histogram overlay or plots that use greyscale to avoid color page charges, FCS Express has you covered.
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Customize Any Plot

All plots can be fully customized in FCS Express allowing you to change backgrounds, dot colors, dot sizes, color schemes, titles, fonts, and more. Customize your plots for day to day flow cytometry analysis so you are ready present or publish at anytime or pick and choose a few representative plots to customize when needed.

Custom Parameter Labels

Often the default parameter label that is saved with your flow cytometry data file is not descriptive enough. FCS Express allows you to use the default labels that exist in the data file or create custom parameter label sets that appear on your plots. Importantly, parameter label sets do not change your original data.

Be a scientist, not a graphic designer

If you ever find yourself using multiple graphic editing packages to get your analysis ready for publication you know how much time and effort can be involved. With FCS Express what you see is what you get. Your figures are ready instantly and better yet, when a simple gate change is needed your formatted plots will update immediately so you don't have to start the process over again.
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Specialized Analysis Plots

When your flow cytometry experiment calls for a specialized analysis technique you can depend on FCS Express for having the right plot for the job. With dedicated plots and analysis tools for cell cycle, proliferation, kinetics analysis, spectral data, and more you will always have what you need at a click.

Avoid Color Page Charges

Not every plot needs to be in full color to convey your results while using black and white or greyscale can save you big money when publishing. FCS Express allows you to work with any plots in greyscale or black and white to get you the visualization you need without breaking the bank when you publish.

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Index Sorting

Use FCS Express to access your flow cytometry index sort data directly from an FCS file

Index sorting allows researchers to review the complete phenotype of every single event sorted into a plate and associate that event with all sorted and non sorted events. Some software packages require you to load .CSV files and run complicated scripts to view the sort data in the context of the plate. FCS Express makes index sorting data analysis simply by utilizing Heat Maps along with the standard plot offerings while loading data as a standard FCS file. No scripting, no csv files, no worries - just results!
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Compatibility

FCS Express supports flow cytometry index sorting data files from all major platforms including Influx (BD Biosciences), Summit Software (Beckman Coulter), Diva (BD Biosciences), and Chorus (BD Biosciences). Simply load your .FCS files and start analyzing

Visualizing Data

Heat Maps allow you to quickly view your entire flow cytometry sort plate in a single click. Overlays allow a user to view up to six statistics and/or parameters on a single heat map so visualizing multi-marker sorts becomes easy. Users may also add a radius statistic, which changes the size of the well to reflect the selected statistic.
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Make the connection from plate to cell

Heat maps will display the number of events and parameter based phenotypic data for all sorted events but wells may be gated on individually, or as groups, allowing users to back gate events onto other plots and quickly find individually sorted cells among the entire phenotype range.

Skip the script

With other flow cytometry software packages you need to use complicated scripts or csv files to access your data. With FCS Express, skip the script and load your data directly into heat maps from your FCS files.

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Publication-ready Histograms

See your flow cytometry data clearly with Histograms

Histograms are an integral aspect of flow and image cytometry data analysis, but researchers often spend too much time and resources in other software packages trying to visualize results and prepare figures for publication. With FCS Express, every aspect of your histograms maybe controlled to arrive at the perfect plot for day-to-day analysis or your next publication in Nature.
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Marker-to-marker Linking

Markers easily are linked to each other by dragging and dropping end points together. Markers can be linked between different histograms or even to gates on 2D plots allowing you the ultimate flexibility for your flow cytometry data analysis.

Overlays made easy

Create histogram overlays with a simple drag and drop. Multiple samples may be overlaid on your histogram to assess different files for the same parameter or compare multiple parameters for one sample. There are no limits on the number of overlays, allowing you to get your flow cytometry results fast.
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Change the perspective

Make your flow cytometry data stand out with three dimensional overlays or use our unique stacked overlay mode to get a clearer picture of your results.

Histogram subtraction

When positives and control populations are close together, the process of dividing positives from negatives can become less of a science and more of an art. FCS Express helps you put the science back again, using the Overton subtraction technique and others to tease apart populations.

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Proliferation Analysis

FCS Express uses a sophisticated fitting process to get results fast

Flow cytometry assays to assess the proliferative activity of cells are commonly used for monitoring the health and growth rate of cells. In this type of assay, a cell tracking dye, such as CFSE, is incorporated into the cells. With each round of cell division, the relative fluorescent intensity of the dye decreases by half. Other software packages offer limited functionality to model the data or require you to use a separate software package completely. With FCS Express full descriptive statistics for modeling and per generation calculations along with the ability to fully format your plots will have you ready for publication in no time in one analysis package.
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Statistics

FCS Express provides a wide range of statistics about the results from the model. It is possible to export any statistic from your proliferation fit to Excel, create PowerPoint slides, save layouts, and more using sophisticated layout tools. Results will also update in real time with gate and data changes so you can get flow cytometry proliferation results from your populations of interest immediately.
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Custom Formatting

FCS Express allows you control every aspect of your proliferation plots so you are ready to present and publish directly from your analysis results.

Flexible Fitting

Custom fitting options allows you to base your starting generation and background noise on controls while other variables can be adjusted to help model your flow cytometry data perfectly every time.

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Intuitive and Familiar Office® Interface

If you can use PowerPoint®, you can analyze in FCS Express

Learning how to use software should not be an impediment to getting results from your data. FCS Express is designed to look, feel, and work like many familiar Microsoft Office programs so you are already half way to being an expert with the software before you even get started. The unique graphical user interface of FCS Express actually represents how your report will be exported to PowerPoint, PDF, or printed. No longer do you have to spend hours learning software, guess how your reports and results will be exported, or sift through endless windows of plots. FCS Express simply helps you move from data to results.
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Familiar ribbon-tab interface

Due to its familiar interface, FCS Express is easy to learn. The customizable ribbon to make it even easier to access the tools you need. Use familiar mouse clicks and keyboard shortcuts to copy, paste, and more. Align, format, group, and order plots and tables as quickly as you can in Microsoft Office®.

Unlimited undo and redo

Mistakes during analysis are inevitable, but you can work boldly with the peace of mind, knowing that you can undo and redo every action you make in your analysis.

Simplify complex workspaces

Don't get lost in dozens of windows and endless open plots. A unified workspace with dockable and pinnable navigators, multiple pages views, and quick access to all aspects of your analysis makes analyzing and exploring the most complex data sets a breeze.
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Export directly to PowerPoint®

Not only does FCS Express function like PowerPoint®, but also it exports directly to PowerPoint® with a single click. PowerPoint exports from FCS Express are high quality; all plots and objects are separate when they arrive in PowerPoint, so you can move, resize, and even ungroup them to be ready to present and publish.

Skip the Copy and Paste

In FCS Express, your analysis is your presentation, figure, or final report. Simply click to export directly to PDF, PowerPoint®, or in the image format of your choice at the resolution you need.

What you see is what you get

Avoid copying and pasting plots, images, or statistics into other software programs for editing and collating. Devote the time previously spent on these tasks to your science because what you see in FCS Express is what you get when exporting your analysis.

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Microsoft Office-like Text Boxes

Organize and present publication-ready results for your daily analysis

It is useful, and often necessary, to collate and organize results from your analysis in one place. FCS Express simplifies the process by harnessing the power of a complete word processor, right inside the program. Adding text and information is as simple as adding a text box and typing just like working in any other PowerPoint presentation or Word document. In FCS Express, you can pull annotations, keywords, system information, and any results from your experiment directly into a text box with the added benefit of real-time updating as gates and data files change. Just like PowerPoint and Word, you will have the ability to format tables, text, and fonts to help make even your daily analysis ready for publication at a click.
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Built-in Text Editor

Emphasize your results by formatting your fonts, adding bullet points, changing line spacing, and other text editing functions with which you know from using Office. The best part is your fully-formatted text boxes and results will update as gates and data files change.

Time-saving Export

Export important information within text boxes by simply dragging and dropping to your batch process. Information will be exported to Excel, LIS/LIMS systems, and any other output you define. Text boxes will also be included in your PowerPoint or PDF exports and can even be copied and pasted when needed. Save time and be more accurate by using text boxes with annotations to avoid manual data entry and possible transcription errors.
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Results Where You Need Them

Text boxes may be inserted anywhere in your analysis, including directly within plots. Placing text boxes in convenient locations allows you to summarize experiments rapidly.

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Spend hours creating reports?

Create multiple documents of your analysis for many files with a single click!

Communication is the key to science and today's researchers need to be able to export results quickly and easily, often times to many different formats. Batch processing in FCS Express makes reporting and sharing your results quick and easy with a variety of export modes and file types. Create images, PDFs, PPTs, XLSs, and more for all your samples by hitting run. Export directly to your LIS/LIMS at same the time, too.
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Generate results faster than ever

One click will send your analysis to PDF, PowerPoint, Excel, LIS/LIMS, and more. You can customize the information included in the reports, where documents are saved, allowing you to always be ready to share your results in moments.

Speed through samples in seconds

Batch process enables analysis of many samples using the same gating strategy. Set up your analysis strategy with necessary samples and let FCS Express iterate through all your files rapidly.

Only export when necessary

FCS Express IVD, you can specify when a report is created with batch processing by defining criteria based on any statistical or data-specific information. Documents meeting the condition are exported without you manually selecting which files for every run.
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Recall gate positions with snapshots

FCS Express automatically records gating, quadrant, and marker position changes between every sample during the batch process. With the batch processing features in FCS Express you will no longer need to make tedious manual adjustments of gates to match those of your exports when rerunning or reviewing an analysis.

Panels

Panels let you quickly and automatically load a set of data files into the correct plots in an analysis. A layout with an active panel can be saved and used over and over again to analyze multiple sets of data for the ultimate efficiency.

What you see is what you get

Avoid copying and pasting plots, images, or statistics into other software programs to create a report. Devote the time previously spent on these tasks to your science because what you see in FCS Express is what you get when exporting your analysis during a batch analysis.

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Real Time Charting

FCS Express offers integrated graphing tools that link directly to your flow cytometry gates

If you find yourself copy and pasting out to Excel and other graphing software with your current analysis tools, only to do it again and again when a gate change is requested, then your not alone. FCS Express eliminates the middle man by utilizing Excel-like spreadsheets built directly into the software that link up with your gates and statistics so you can skip the copy and paste and get results in real time.
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Pie Charts

Pie Charts facilitate the visualization of relationships between statistics and how they combine to form a conceptual whole.  Rather than export your flow cytometry data to a spreadsheet or other graphing program, simply insert a Pie Chart in FCS Express and watch as it updates automatically with your gates, markers, quads, and .fcs file data.

Bar Charts

Bar Charts update immediately as your analysis changes including standard deviation bars. Bar Charts are fully formattable within FCS Express, eliminating the need to use additional software programs to achieve your results.

Scatter Plots

FCS Express has built-in Scatter Plots that update in real time when gates are adjusted. Data from Scatter Plots can be overlaid from the same or multiple flow cytometry experiments. Scatter Plots are fully formattable, including dot, error bar, and all other plot properties.
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Scatter with Regression Plots

FCS Express can display your flow cytometry data as a Scatter with Regression Plot when performing a time-course study, dose-response curve, or other quantitative experiment. Choose from many available equations to fit your data and watch the curve update in a batch process or as you adjust your gates!

Regression Analysis

FCS Express enables the calculation of regression fits directly from your gates and statistics. You can overlay a regression with the click of a button. Most importantly, your regression formulas, line fits, and analysis can all be linked directly to your flow cytometry data allowing you to view changes to your regression analysis in real time as gates and data are adjusted.

Levey-Jennings Plots

With Levey-Jennings plots, you may visualize quality control data to ensure test metrics are stable over time and fall within a predetermined range. The range, more commonly referred to as the control limits, allows the test metric to be assessed based on whether the values fall within the range or outside of the range. Values falling outside of the control limits are considered "out of control" and when occurring on consecutive measurements can indicate the accuracy of the test metric has shifted.

Other Summary Plots

Box and Whisker, Violin, and Beeswarm Plots are new additions to enable you to see the central tendency and distribution of your data.

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Easily Access, Edit, and Use Keywords

FCS Express provides easy access to the information stored within your .FCS files.

A flow cytometry FCS file contains a section known as the header which contains information about the data, including the names of the parameters, the amplification method, acquisition date and time and many other information. With other software packages accessing this information can be tricky and using it annotate your analysis can be even harder. FCS Express allows you to view, change, and use any of the information contained in the header and to save the changed information in with the file.
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Add or Modify Keywords with Ease

It is easy to add additional keywords in FCS Express. Simply use the Add Keyword option or create an excel table with your new keywords and apply it to your flow cytometry experiment. FCS Express has options for adding or changing annotations and keywords for one, some, or all of your files in an experiment.

Change Annotations at Any Time

If you entered the wrong name for the operator, parameter, stain, or other meta-data at the time of acquisition you can simply change it. After editing simply save the file with a new name and continue on with analysis.
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Anonymize Your Data

At times it is necessary to strip any identifying information from your flow cytometry data files. With FCS Express you can remove keywords one by one or use the convenient anonymizing tool to remove identifying information from one or many files with a click.

Sort Data By Keywords

Columns for sorting data by specific keywords and meta-data are accessible to you at any time. Want to quickly arrange your flow cytometry data files for analysis by the time they were acquired or the tube number? Simply use the sorting features in FCS Express to organize your analysis.

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Streamline your process

Get your flow cytometry results transferred to the LIS faster.

Eliminate tedious and error-prone manual data entry with custom solutions for automated LIS integration in FCS Express.
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Save time and money

Skip manual data entry!  With the click of a button, send all your demographics, statistics, canned comments, and more to your LIS instantly.

Export only what you need

Custom solutions allow you to transfer important results in the format you need.  Take advantage of customized solutions to export results directly from FCS Express to your LIS. 
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Launch the right analysis every time

Take your automation to the next level with launch files.  Open the layout you need with the correct data files.  Eliminate wasted time searching for data files or loading the incorrect layout.

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