Integrated Spreadsheets

Imagine having the power of Excel with results linked directly to your flow cytometry gates

Flow cytometry data analysis rarely ends with plots and raw statistics so you probably spend a lot of time copy and pasting from your current analysis package to Excel and other charting software to generate your final result. Then what happens when you need to change a gate? You have to do it all over again. With FCS Express spreadsheets can be accessed directly in your analysis and linked up to your gates and data files. As gates move and data changes, so do your spreadsheets in real time.  Say good bye to copy and paste and hello to results!

Statistics made easy

Values in Spreadsheet cells may be derived directly from any gate or statistic, keywords, or from all your flow cytometry data files with one drag and drop. Spreadsheet cells are easily filled by dragging and dropping your data files or statistics right into the spreadsheet.

Formatting

Spreadsheets in FCS Express share many of the common formatting features of Excel so you will be able to sort, merge, use conditional formatting, insert/remove columns/rows, format text, and much more without having to open two software packages or copy and paste.

Formulas

Almost all of the of the formula features and operations that Microsoft Excel provides are included in FCS Express with the added advantage that formula results can be directly linked to any plot or gate based statistic. Imagine the power of moving a gate and having p-values, t-tests, and other advanced statistics from your flow cytometry data analysis update immediately.

Charts

Data in spreadsheets can also be visualized in Pie charts, Bar plots, Scatters plots, and Regression charts with a click. FCS Express eliminates the middle man of copy and pasting to Excel by linking up with your gates and statistics so you can get results in real time.

Comprehensive Statistics

The right statistics where you need them when you need them

Today's researchers need to have access to statistics and results from data as quickly and easily as possible. While standard statistics like % Gated and MFI are important, researchers generally rely on multiple software packages outside of their flow analysis software to perform simple to complex calculations in spreadsheets and other graphing tools. FCS Express comes packed with all of the standard descriptive statistics you have come to expect with the added ability to use spreadsheets directly within the software. Using statistical analysis tools in FCS Express allows you to see your results update as gates and data files change while skipping the copy and paste out to other packages

Spreadsheets linked to your data

Perform powerful statistical analysis and generate custom formulas with our Integrated Spreadsheets. Spreadsheets can perform most of the operations found in Microsoft Excel. Importantly the values in Spreadsheets are linked directly to your data, so when you move your gates or change data files the Spreadsheet values will update in real-time.

Custom Statistics

Create equations, functions, and statistics that update in real time based on your analysis. Eliminate manual calculations for better efficiency, accuracy, and standardization. With FCS Express, there’s no need to move your complex data analysis to outside programs. You can do it all right within FCS Express.

Results where you need them

FCS Express allows you to integrate your statistics anywhere on your layout. From plots to custom tables and text boxes, you can format and display your results the way you want. Make your layouts and images publication ready with FCS Express!

Regression Analysis and Statistical Tests

Perform statistical testing and regression analysis all within FCS Express. From the T-Test, Chi-Squared, Z-Prime, to Michaelis-Menten and Logistic Regression, see what FCS Express can do for your data analysis.

Specialized Analysis Tools and Statistics

Working with kinetics data? Modeling cell cycle or proliferation results? Need to calculate the concentration of your gated cells? Our specialized analysis tools come with a complete set of statistical reporting and output options. Make complex tasks easy with FCS Express!

Plots with Flexible Formatting

FCS Express has the visualizations you need to move from flow cytometry analysis to publication

Communicating your results visually is an important aspect of any analysis, report, or publication. With other cytometry analysis packages researchers tend to rely heavily on external software tools to prepare their plots and figures for presentation and publication. With FCS Express every aspect of your plots can be easily customized directly in the software so you arrive at the visualization needed during analysis. Whether you are looking for a colorful backgated plot or histogram overlay or plots that use greyscale to avoid color page charges, FCS Express has you covered.

Customize Any Plot

All plots can be fully customized in FCS Express allowing you to change backgrounds, dot colors, dot sizes, color schemes, titles, fonts, and more. Customize your plots for day to day flow cytometry analysis so you are ready present or publish at anytime or pick and choose a few representative plots to customize when needed.

Custom Parameter Labels

Often the default parameter label that is saved with your flow cytometry data file is not descriptive enough. FCS Express allows you to use the default labels that exist in the data file or create custom parameter label sets that appear on your plots. Importantly, parameter label sets do not change your original data.

Be a scientist, not a graphic designer

If you ever find yourself using multiple graphic editing packages to get your analysis ready for publication you know how much time and effort can be involved. With FCS Express what you see is what you get. Your figures are ready instantly and better yet, when a simple gate change is needed your formatted plots will update immediately so you don't have to start the process over again.

Specialized Analysis Plots

When your flow cytometry experiment calls for a specialized analysis technique you can depend on FCS Express for having the right plot for the job. With dedicated plots and analysis tools for cell cycle, proliferation, kinetics analysis, spectral data, and more you will always have what you need at a click.

Avoid Color Page Charges

Not every plot needs to be in full color to convey your results while using black and white or greyscale can save you big money when publishing. FCS Express allows you to work with any plots in greyscale or black and white to get you the visualization you need without breaking the bank when you publish.

Publication-ready Histograms

See your flow cytometry data clearly with Histograms

Histograms are an integral aspect of flow and image cytometry data analysis, but researchers often spend too much time and resources in other software packages trying to visualize results and prepare figures for publication. With FCS Express, every aspect of your histograms maybe controlled to arrive at the perfect plot for day-to-day analysis or your next publication in Nature.

Marker-to-marker Linking

Markers easily are linked to each other by dragging and dropping end points together. Markers can be linked between different histograms or even to gates on 2D plots allowing you the ultimate flexibility for your flow cytometry data analysis.

Overlays made easy

Create histogram overlays with a simple drag and drop. Multiple samples may be overlaid on your histogram to assess different files for the same parameter or compare multiple parameters for one sample. There are no limits on the number of overlays, allowing you to get your flow cytometry results fast.

Change the perspective

Make your flow cytometry data stand out with three dimensional overlays or use our unique stacked overlay mode to get a clearer picture of your results.

Histogram subtraction

When positives and control populations are close together, the process of dividing positives from negatives can become less of a science and more of an art. FCS Express helps you put the science back again, using the Overton subtraction technique and others to tease apart populations.

Microsoft Office-like Text Boxes

Organize and present publication-ready results for your daily analysis

It is useful, and often necessary, to collate and organize results from your analysis in one place. FCS Express simplifies the process by harnessing the power of a complete word processor, right inside the program. Adding text and information is as simple as adding a text box and typing just like working in any other PowerPoint presentation or Word document. In FCS Express, you can pull annotations, keywords, system information, and any results from your experiment directly into a text box with the added benefit of real-time updating as gates and data files change. Just like PowerPoint and Word, you will have the ability to format tables, text, and fonts to help make even your daily analysis ready for publication at a click.

Built-in Text Editor

Emphasize your results by formatting your fonts, adding bullet points, changing line spacing, and other text editing functions with which you know from using Office. The best part is your fully-formatted text boxes and results will update as gates and data files change.

Time-saving Export

Export important information within text boxes by simply dragging and dropping to your batch process. Information will be exported to Excel, LIS/LIMS systems, and any other output you define. Text boxes will also be included in your PowerPoint or PDF exports and can even be copied and pasted when needed. Save time and be more accurate by using text boxes with annotations to avoid manual data entry and possible transcription errors.

Results Where You Need Them

Text boxes may be inserted anywhere in your analysis, including directly within plots. Placing text boxes in convenient locations allows you to summarize experiments rapidly.

Easily Access, Edit, and Use Keywords

FCS Express provides easy access to the information stored within your .FCS files.

A flow cytometry FCS file contains a section known as the header which contains information about the data, including the names of the parameters, the amplification method, acquisition date and time and many other information. With other software packages accessing this information can be tricky and using it annotate your analysis can be even harder. FCS Express allows you to view, change, and use any of the information contained in the header and to save the changed information in with the file.

Add or Modify Keywords with Ease

It is easy to add additional keywords in FCS Express. Simply use the Add Keyword option or create an excel table with your new keywords and apply it to your flow cytometry experiment. FCS Express has options for adding or changing annotations and keywords for one, some, or all of your files in an experiment.

Change Annotations at Any Time

If you entered the wrong name for the operator, parameter, stain, or other meta-data at the time of acquisition you can simply change it. After editing simply save the file with a new name and continue on with analysis.

Anonymize Your Data

At times it is necessary to strip any identifying information from your flow cytometry data files. With FCS Express you can remove keywords one by one or use the convenient anonymizing tool to remove identifying information from one or many files with a click.

Sort Data By Keywords

Columns for sorting data by specific keywords and meta-data are accessible to you at any time. Want to quickly arrange your flow cytometry data files for analysis by the time they were acquired or the tube number? Simply use the sorting features in FCS Express to organize your analysis.

Tokens Get You Results

Tokens are live updating text, statistics, and system information from your analysis

Making sure your analysis is annotated correctly and organizing your statistics is crucial for all scientists. FCS Express allows you to insert live-updating text called Tokens anywhere in your analysis. They can display plot statistics, file keywords, gate and layout properties, and even system information from your computer. Tokens will accelerate your time to results.

Keep Track of Your Experiments

Tokens allow you to access the date and time that your files were acquired and other information that you entered at the time of acquisition. With tokens you will never need to enter information manually, or copy and paste, if you have entered it when you acquired your files. Never sift through your lab note book for important dates and experiment information again.

Incorporate Stats Directly in Your Plots

Tokens can be dynamically incorporated into plots to quickly summarize your analysis in place or to create stunning figures for publication and collaboration.

Tokens are as Easy as a Drag and Drop

Easily add tokens anywhere by dragging and dropping. Have a plot with a gate on it? Drag and Drop the gate to insert a token for MFI. Have a data file open? Drag and Drop to access keywords and show the Tube Number. Need statistics from your stats tables somewhere else? Drag and Drop to place the statistics anywhere in the layout.

Customize and Create

Custom Tokens can help you create a custom value for any mathematical expression, allow you to perform conditional IF/THEN statements, convert numeric values into simple text such as Positive or Negative all while remaining live updating so as you move a gate, your custom values update immediately.

Tokens in the Clinical Setting

FCS Express IVD incorporates special tokens that are critical for Clinical labs. These tokens can be triggered by any numeric or text values and allow for real time Statistical Classifiers, Font Formatting, Page Visibility, and Batch Process Action triggering.

Publication Quality Graphics

Eliminate the need to use multiple software packages to present your data

Generating high quality graphics and reports to communicate your results can be hard with other software packages and may involve using many different image editing tools. When you are ready to publish and present your data FCS Express has you covered by supporting direct high-resolution exports to PowerPoint, PDF, and six image formats at up to 750 DPI resolution. Never fight with low resolution graphics and bulky workarounds again when all you want are results!

Skip the Copy and Paste

In FCS Express your analysis is your presentation, figure, or final report. Simply click to export directly to PDF, PowerPoint, or in the image format of your choice at the resolution you desire.

Full Reports at High Resolution

Your analysis can be set up as a full report including high resolution graphics. Your reports are linked up directly to your data so as you move a gate your report updates immediately. When you are ready to publish, simply export to the format of your choice.

High Quality PowerPoint Presentations

]If you have ever struggled with low quality PowerPoint exports from your current software then FCS Express is for you. Your analysis and plots are exported from FCS Express at the highest resolution possible and individual plots and objects will arrive in PowerPoint also as individual objects so you can move, resize, and format all objects independently in PPT if needed.

Big Experiments? Big Data? No Problem

You never have to sacrifice the quality of your reports and figures due to the size of your experiments or number of data files with FCS Express. The easy to use batch processing feature in FCS Express allows you to export at the same quality across any number of samples, to multiple formats (PowerPoint, Word, Excel, PDF, Images) simultaneously, all with the click of a button.
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