Pre-defined Algorithms

Easy to Use Pre-assembled Tools for Your Analysis

Researchers wanting to use the latest methods to prepare and analyze their data will no longer be hindered by complex programming requirements. FCS Express offers Pre-Defined algorithms for FlowAI, FlowSOM, or SPADE in our new Pipelines. Skip the complex scripts, stop reaching for R, or worrying about programming. With these Pre-Defined algorithms in FCS Express you are only a few click away from preparing your data files and performing advanced analysis - no plugins required.


FlowAI an automatic and interactive tool to remove outliers and anomalies from your data files is readily available for your use in FCS Express.  This pre-defined pipeline step includes a flow rate check, signal acquisition, and dynamic range quality controls. Removing these lesser quality events allows for improved downstream basic and advanced analysis while generating superior, reliable, and reproducible results in FCS Express.


Now accessible through FCS Express Pipelines as a pre-defined algorithm, FlowSOM is a clustering and visualization tool that facilitates the analysis of high-dimensional data. Clusters are arranged via a Self-Organizing Map (SOM) in a Minimum Spanning Tree, that can help Researchers understand how markers included in their panel are behaving on all cells.  A second clustering step (i.e., meta-clustering) is performed, which can provide a basis for discerning biological similarity and can help detect groups that may have otherwise been missed.


SPADE (Spanning-tree Progression Analysis of Density-normalized Events) is a clustering algorithm that is complementary to existing analysis approaches available in FCS Express. As a pre-defined pipeline step, it is easy for FCS Express users to automatically identify cell populations in multidimensional cytometry data files without needing to assign a known cellular ordering.