System Requirements

System Requirements and File Compatibility

File Compatibility

FCS Express can read listmode (FCS) data files from all manufacturers. FCS Express currently supports FCS Version 1 to FCS Version 3.2*.

FCS Express can read data files that were acquired on a Macintosh or Windows operating system. Simply transfer the files to the computer on which you are running FCS Express (either via the network, cloud storage, flash drive, CD, or any other method) and FCS Express will be able to load the data.

In addition to being able to load the raw listmode data from any instrument, FCS Express can also import the analysis/acquisition settings (gates, plots, markers, quadrants, etc) from the BD FACSDiva™ and the BD Accuri C6™ flow cytometers.

*Please note that FCS 3.2 compatibility will be found in FCS Express 7.18.0021 or higher.

To learn more, expand a section below to learn the requirements for each component.

System Requirements

Operating System

Windows:
- FCS Express 6 and 7 can run on Windows 7/Windows 10/Windows 11.
- 32-bit FCS Express will run in a 32-bit or 64-bit environment.
- 64-bit FCS Express will run in a 64-bit environment.
- Why choose 64-bit over 32-bit?
  - Computers running 64-bit applications generally have more resources, such as power or memory, that are available to them, compared to their 32-bit counterparts.
  - If you are analyzing large data files or large datasets, and your computer is running a 64-bit operating system, a 64-bit application is recommended to maximize the amount of memory that can be allocated to an application.
MacOS:
- The latest release of FCS Express 7 is compatible with macOS 10.13 (High Sierra) and later.
- FCS Express Version 6 is not compatible with macOS Catalina (10.15) or higher.
- FCS Express for MacOS is not compatible with our Network license.
- Note about future macOS releases:
  - macOS 27 Golden Gate
    - FCS Express is compatible with macOS 27 Golden Gate.
    - macOS 27 Golden Gate does not include Rosetta, which is the translation layer that allows Intel-compiled software to run on Apple Silicon (M-series) hardware.
    - When launching FCS Express for the first time after upgrading to Golden Gate, the follow message appears:

- - - Click Install and macOS will install Rosetta.
    - Launch FCS Express again and the app should launch.
  - macOS 28
    - De Novo Software is aware that Apple has announced that starting with macOS 28, Macs will no longer support Rosetta.
    - macOS 28 is expected to be released in Fall 2027, and Rosetta will remain available and supported through macOS 27, giving the De Novo Software Team ample time to prepare for a transition.
    - De Novo Software intends that FCS Express will remain compatible with future macOS releases.
    - We are actively working to ensure that FCS Express is compatible with an Apple Silicon build well ahead of the macOS 28 deadline.
    - If you have any questions about compatibility or your current installation, please contact us at support@denovosoftware.com.

Processor

There is no minimum processor requirement.
However, the "better" the processor, the more responsive FCS Express will become. This is important to consider, especially if your analysis will consist of:
- large datasets
- spectral data
- high dimensional data reduction tools, such as tSNE, FlowSOM, UMAP, etc.
For starters, a "better" processor will consist of:
- more cores,
- more logical processor capability,
- an increased clock speed, or frequency, of the CPU. Many of the modern processors on the market will have a frequency measured in gigahertz or GHz.
Please note that a higher spec'd CPU will be advantageous if the live updating feature is enabled.
FCS Express takes advantage of many features that are available in higher-end Intel Chips. For optimum performance, use the newest generation processors. For example, an i7 will significantly outperform an i5 even if the number of cores and clock speed of the processors are equivalent. Also, a Desktop processor can perform better than a Laptop processor.
FCS Express can take advantage of all cores in the computer. While not required, a multi-core computer will be significantly faster than a single-core computer for many functions.

Memory

FCS Express itself requires very little memory. The amount of memory you need is determined primarily by the size and number of data files that you are going to be analyzing at the same time. As a general rule, we suggest as much memory as possible.
FCS Express (32-bit) (PC Only) can use up to 3 GB of memory on a 32-bit Windows system, and 4 GB of memory on a 64-bit Windows system.
FCS Express (64-bit) (PC and Mac) can use up to 128 GB on 64-bit machine.
Please visit our detailed memory requirements page for details on out of memory messages and details on how to allocate more memory.

Hard Drive Space

Internet Connectivity

Permissions

For Standalone installations of FCS Express, a standard user will need to Read and Write access to:

Windows:

C:\ProgramData\De Novo Software\<FCS Express Version Number and Edition>

macOS

~Library/De Novo Software/<FCS Express Version Number and Edition>

For Network installations, the Window User will Read/Write permissions to the folder in which FCS Express is installed on the server or file share.

Network Version Requirements

There is no particular system requirement for the app server or file share where FCS Express Network will be installed. The server or file share simply acts as a repository for the FCS Express installation.

The app server or file share just needs enough space to install the app and whatever background apps the repository needs. Since the software executes on the client's local computer, it does not consume resources of the file server.

The FCS Express network version has been successfully installed on Windows based, Linux based and SAN networks.

Security System

Microsoft Office

PowerPoint and Excel are registered trademarks of Microsoft Corporation

FACSDiva is a trademark of Beckton Dickinson Corporation

System Requirements and File Compatibility

File Compatibility

System Requirements

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