This section focuses on how to create, apply and manipulate a compensation matrix within FCS Express.

• Web Page - Compensation in FCS Express
• User Manual - Compensation and Spectral Unmixing
• Ask an Expert Compensation Live Stream
• Compensation Webinar
• Compensation Tutorial

Compensation is the process of removing the unwanted fluorophore signal from secondary channels. This page provides useful background information to help users understand how compensation is being implemented within FCS Express.

• Web Page - Compensation Implementation

In flow cytometry, compensation is a topic that can stir a heated debate about how to best implement this important concept into your workflow.

Please use this link as a resource to help you figure out the workflow that best fits your situation.

• Knowledge Base - Compensation Workflow

Even though FCS Express works under the default assumption that your compensation control files are of a single media type, you can easily create a matrix in FCS Express that uses cells and beads for controls. Data Specific Gates in FCS Express makes this an easy application. This brief video will show you how to do this.

• Performing Mixed Media Compensation Quick Video