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1Introduction to Overlays in FCS Express

This section focuses on how to create, apply and manipulate overlays within FCS Express.

2Legends

Legends are useful to describe the data presented in your plots and to help distinguish between several data files that are overlaid onto a single plot and can be easily enabled within the analysis.

3The Histogram Subtraction Tool

Histogram subtraction allows you to determine the percent positive on a histogram by subtracting a negative control histogram from a sample histogram. Histogram subtraction can be performed either on an individual basis, or set up to be automatically calculated and appear on the statistics window for a series of histograms.