Application Examples

Flow Cytometry Bead Based Assays to Calibrate and Produce High Quality and Reproducible Results

In This Video

In this webinar the teams at Spherotech and De Novo Software have collaborated to teach you about the beads and workflows you can use to perform daily QC checks on your cytometers, calibrate your data sets to MESF, and use FCS Express to do so in an mostly automated fashion without external Excel spreadsheets. The team from Spherotech discusses the technology and current workflows for using their Ultra and Rainbow Calibration Particles and the team from De Novo Software follows to teach researchers how to use FCS Express for quick reporting and tracking of QC results, utilize regression graphing that links directly to your data sets/gates, and automatic channel calibration from known MESF standards.

Learn How To

Prepare Particles and Set Acceptable Ranges
Utilize Templates for Bead Calibration Assay Analysis
Incorporate Integrated Spreadsheets and Charts for Reporting

Immunophenotyping

In This Video

Join us as we analyze an immunophenotyping experiment in FCS Express. Watch as we insert and format plots, apply gates, markers and quadrants; add statistics to our layout; and, batch export our results to PowerPoint and Excel. We’ll then add more tubes to our experiment and analyze them side by side, using Panels. Rather than focusing on any particular procedure, we’ll aim to quickly become familiar with many of the basic yet powerful features of FCS Express—all in the context of a natural workflow for analyzing flow cytometry data.

Learn How To

Establish a Data Analysis Strategy
Build a Gating Hierarchy and Insert Spreadsheets
Use Batch Processing to Export Results

Index Sorting Data Analysis

In This Video

Investigate how index sorting allows researchers to review the complete phenotype of every single event sorted into a plate and associate that event with all sorted and non sorted events. FCS Express 7 allows for analysis and reporting of index sorting data using Heat Maps at a click along with the standard plot offerings.

Learn How To

Insert a Plate Heat Map and View Index Sorted Data
Create Gates Based on Wells of the Heat Map
Format Heat Maps and Extract Relevant Statistics

Performing Antibody Titration

In This Video

Learn why titration is an important step in the panel optimization process. Improperly titrated reagents can lead to increases in fluorescence background and poor overall resolution of your flow cytometry data. This webinar will help teach you the concepts that you can use in FCS Express to analyze your titration data. This webinar will also introduce the release of our Universal Antibody Titration Template that can be used for 5, 6, or 7 point titration experiments.

Learn How To

Add Titration Data to the Analysis Workspace
Use the Universal FCS Express Titration Template
Build a Titration Template

Stain Index Analysis

In This Video

With FCS Express, learn how you can easily create your own Stain Index analysis and utilize your data set to obtain the best possible results.

Learn How To

Calculate Stain Index Using Integrated Spreadsheets
Calculate Stain Index Using Custom Tokens
Insert Regression Plot to Derive Statistics

Dotmatics Biology Solution

In This Video

The Dotmatics Biology Solution supports drug discovery across a wide range of biologic therapeutics including antibodies, CAR-T cells, and RNA therapeutics. The solution provides end-to-end biologics data management and analysis capabilities that fit into established complex workflows, while the platform enables structured data for users to easily access, collaborate, visualize, and analyze results.

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