Compensation and Spectral Unmixing

EasyPanel Integration

In This Video

Learn how the integration between FCS Express and EasyPanel for spectral data works. This integration allows you to validate the quality of the reference signatures of your unmixing matrices, and also to upload said signatures, including autofluorescence, to leverage the EasyPanel algorithm for automated panel design.

Learn How To

Export the spectral signatures of your reference controls from FCS Express to EasyPanel for comparison to expected signatures.
Set your own controls as the benchmark for comparison.
Automatically design a panel accounting for autofluorescence.

Compensation (Short video)

In This Video

Learn how to use the automatic compensation wizard in FCS Express and how to save, move, and apply compensations between different samples and layouts.

Learn How To

Adjust Compensation Behaviors
Access and Edit the Compensation Matrix
Create a New Compensation Matrix

Compensation (Full video)

In This Video

Explore how FCS Express reads acquisition-derived compensation from any modern flow cytometer. You may also edit or create compensation matrices within the software. During the webinar you will learn how to use the auto-compensation tools of FCS Express, how to adjust compensation manually as needed, and how to ensure the intended compensation matrix or matrices are applied to your plots in a during the Batch Process. We will also show you new compensation-related features introduced with FCS Express 7, such as NxN plots, Spillover Spreading Matrix, Cross Staining Index, and the new “real time” updating compensation introduced in 7.08.0018.

Learn How To

Use the Auto-Compensation tools of FCS Express
Adjust Compensation Manually
Apply Compensation to Data

Mixed Media Compensation

In This Video

Learn how to perform Compensation with mixed media data sets. FCS Express works under the default assumption that your compensation control files are of a single media type, but you can easily create a matrix in FCS Express that uses cells and beads for controls. Data Specific Gates in FCS Express make it easy to create gates for beads and cells as needed.

Learn How To

Add a New Compensation
Create a Data Specific Gate for Additional Control
Import a Compensation Matrix

Similarities and differences of performing compensation in FCS Express and FlowJo

In This Video

In continuation of our series, Transitioning to FCS Express, we explore Compensation similarities and differences to FlowJo.

Learn How To

Compare how FCS Express and FlowJo perform compensation
Transition from FlowJo to FCS Express

Spectral Unmixing (Short video)

In This Video

Make the most of Spectral flow cytometry and it’s ability to increase the number or parameters per single cell or particle than traditional flow cytometry. In contrast to conventional flow cytometers, spectral flow analyzers and sorters substitute mirrors, optical filters, and photomultiplier tubes (PMTs) with a spectrograph and multichannel detector to use fluorescence or Raman spectroscopy. This video will quickly show you how to unmix your spectral flow cytometry data in FCS Express.

Learn How To

Add Unmixing Controls
Select a Sample Type for a Control
Export an Unmixing Matrix

Spectral Unmixing (Full video)

In This Video

In this webinar the team at De Novo Software will guide you through the process of unmixing using the new Spectral Unmixing wizard in FCS Express 7. We will teach you how unmix your spectral data sets from single stained controls, apply the unmixing to sample data files, and proceed to generate high quality and publication ready results!

Learn How To

Explore 3 Different Unmixing Examples
Apply Unmixing to Plots
Save an Unmixing and Utilize in New Layout

Spectral Flow Cytometry Analysis Techniques for FCS Express

In This Video

Learn more about the technology and methodology behind Spectral Flow Cytometry and how FCS Express 7 provides a native support for spectral data files with linking to the raw spectral data as well as standard flow cytometry files, plots, and methods. The seamless integration allows you to move from acquisition to results quickly and easily while retaining access to spectral information and advanced batch processing, custom calculation, plate based, and dimensionality reduction transformation tools.

Learn How To

Use Unmixed Data Files
Create an Unmixing Matrix
Perform Virtual Unmixing

Virtual Spectral Unmixing

In This Video

Learn how FCS Express allows you to visualize, unmix and treat any over-determined data files as a “Virtual Spectral” data file via the addition of special keywords to the data set. In this webinar, we cover the basics of creating a virtual spectral data file, how to perform spectral unmixing from these data types, and how to gather results from your virtual spectral data sets.

Learn How To

Create a Virtual Spectral Data File
Perform Spectral Unmixing from Virtual Spectral Data File
Get results from Virtual Spectral Data Sets

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