Spectral Cytometry

New Compatibilities for Spectral and Image Cytometry Instruments

In This Video

FCS Express version 7.32 has expanded compatibility with FACSDiscover S8/A8, Xenith and Cytek EVO data. FCS Express® supports instrument-specific settings and data visualization options that automatically adapt to different file formats and acquisition systems. These features help researchers streamline workflows, maintain consistency, and maximize insights across experiments. They enable users to move seamlessly between platforms while preserving data fidelity and analysis quality, improving efficiency, and enhance collaboration across the lab.

Learn How to

Work with data from BD FACSDiscover, Thermo Fisher Xenith™ and Cytek EVO™ instruments in FCS Express®
Use practical strategies for analyzing, comparing, and reporting results from diverse instruments within a single software solution

EasyPanel Integration

In This Video

Learn how the integration between FCS Express and EasyPanel for spectral data works. This integration allows you to validate the quality of the reference signatures of your unmixing matrices, and also to upload said signatures, including autofluorescence, to leverage the EasyPanel algorithm for automated panel design.

Learn How To

Export the spectral signatures of your reference controls from FCS Express to EasyPanel for comparison to expected signatures.
Set your own controls as the benchmark for comparison.
Automatically design a panel accounting for autofluorescence.

Spectral Unmixing (Short video)

In This Video

Make the most of Spectral flow cytometry and it’s ability to increase the number or parameters per single cell or particle than traditional flow cytometry. In contrast to conventional flow cytometers, spectral flow analyzers and sorters substitute mirrors, optical filters, and photomultiplier tubes (PMTs) with a spectrograph and multichannel detector to use fluorescence or Raman spectroscopy. This video will quickly show you how to unmix your spectral flow cytometry data in FCS Express.

Learn How To

Add Unmixing Controls
Select a Sample Type for a Control
Export an Unmixing Matrix

Spectral Unmixing (Full video)

In This Video

In this webinar the team at De Novo Software will guide you through the process of unmixing using the new Spectral Unmixing wizard in FCS Express 7. We will teach you how unmix your spectral data sets from single stained controls, apply the unmixing to sample data files, and proceed to generate high quality and publication ready results!

Learn How To

Explore 3 Different Unmixing Examples
Apply Unmixing to Plots
Save an Unmixing and Utilize in New Layout

Spectral Flow Cytometry Analysis Techniques for FCS Express

In This Video

Learn more about the technology and methodology behind Spectral Flow Cytometry and how FCS Express 7 provides a native support for spectral data files with linking to the raw spectral data as well as standard flow cytometry files, plots, and methods. The seamless integration allows you to move from acquisition to results quickly and easily while retaining access to spectral information and advanced batch processing, custom calculation, plate based, and dimensionality reduction transformation tools.

Learn How To

Use Unmixed Data Files
Create an Unmixing Matrix
Perform Virtual Unmixing

Virtual Spectral Unmixing

In This Video

Learn how FCS Express allows you to visualize, unmix and treat any over-determined data files as a “Virtual Spectral” data file via the addition of special keywords to the data set. In this webinar, we cover the basics of creating a virtual spectral data file, how to perform spectral unmixing from these data types, and how to gather results from your virtual spectral data sets.

Learn How To

Create a Virtual Spectral Data File
Perform Spectral Unmixing from Virtual Spectral Data File
Get results from Virtual Spectral Data Sets

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