Compensation

EasyPanel Integration

In This Video

Learn how the integration between FCS Express and EasyPanel for spectral data works. This integration allows you to validate the quality of the reference signatures of your unmixing matrices, and also to upload said signatures, including autofluorescence, to leverage the EasyPanel algorithm for automated panel design.

Learn How To

Export the spectral signatures of your reference controls from FCS Express to EasyPanel for comparison to expected signatures.
Set your own controls as the benchmark for comparison.
Automatically design a panel accounting for autofluorescence.

Compensation (Short video)

In This Video

Learn how to use the automatic compensation wizard in FCS Express and how to save, move, and apply compensations between different samples and layouts.

Learn How To

Adjust Compensation Behaviors
Access and Edit the Compensation Matrix
Create a New Compensation Matrix

Compensation (Full video)

In This Video

Explore how FCS Express reads acquisition-derived compensation from any modern flow cytometer. You may also edit or create compensation matrices within the software. During the webinar you will learn how to use the auto-compensation tools of FCS Express, how to adjust compensation manually as needed, and how to ensure the intended compensation matrix or matrices are applied to your plots in a during the Batch Process. We will also show you new compensation-related features introduced with FCS Express 7, such as NxN plots, Spillover Spreading Matrix, Cross Staining Index, and the new “real time” updating compensation introduced in 7.08.0018.

Learn How To

Use the Auto-Compensation tools of FCS Express
Adjust Compensation Manually
Apply Compensation to Data

Mixed Media Compensation

In This Video

Learn how to perform Compensation with mixed media data sets. FCS Express works under the default assumption that your compensation control files are of a single media type, but you can easily create a matrix in FCS Express that uses cells and beads for controls. Data Specific Gates in FCS Express make it easy to create gates for beads and cells as needed.

Learn How To

Add a New Compensation
Create a Data Specific Gate for Additional Control
Import a Compensation Matrix

Similarities and differences of performing compensation in FCS Express and FlowJo

In This Video

In continuation of our series, Transitioning to FCS Express, we explore Compensation similarities and differences to FlowJo.

Learn How To

Compare how FCS Express and FlowJo perform compensation
Transition from FlowJo to FCS Express

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