FCS Express has been specially optimized for use with FCS Data files generated by Abbott's Cell-Dyn Sapphire® instrument. When the Sapphire software exports results from the instrument, it packages all of the separate cell populations into a single compound file. Most flow cytometry data analysis software cannot even open the compound file, but all you have to do in FCS Express is change a few settings to make Abbott files as easy as any other. See our poster from the 2005 ISLH XVIII meeting.
Below is an explanation of the different data sets in a typical Sapphire data file.
|Data Set Name||Explanation|
|WBCod||white blood cell optical data|
|RBCi||Red blood cell impedance data|
|PLTi||Platelet impedance data|
|PLTo||optical platelet data|
|HGBr||optical density readings for the hemoglobin measurement|
|HGBs||optical density readings for the hemoglobin measurement|
|CD3_8||CD 3/8 analysis|
|CD61||CD61 (immunoplatelet analysis)|
We have several resources available for Sapphire users.
- Detailed instructions on downloading and installing FCS Express.
- Detailed information on setting up FCS Express to work with the Cell-Dyn Sapphire.
- Brief tutorial on using FCS
Express to analyze immunofluorescence data from the Sapphire.