Knowledgebase: How can I display multiple regression curves or reference data points on a single regression plot?

FCS Express 6 allows for the display of additional regression curves or data points onto your regression plots.

overlaid Scatter with Regression curves
(e.g., multiple antibody titrations,
drug IC50 curves)

overlaid data points
(e.g., interpolated concentrations,
theoretical reference values)

The expandable sections below outline the procedure for creating each kind of overlay.

Overlaid Scatter with Regression Curves

By using a Scatter plot as your base overlay, multiple Scatter with Regression curves may be visualized as overlays within one plot. This is useful for plotting multiple antibody titrations, drug IC50 curves, etc.

All plots and Spreadsheets referred to below must be visible on the same page of your layout.

  1. Create one Scatter with Regression Plot for each regression curve that you need.
  2. Insert a new, empty Spreadsheet (Insert tab> Spreadsheet> New Spreadsheet).
  3. Insert a Scatter Plot (not a Scatter with Regression Plot), while the new Spreadsheet is selected. The Scatter Plot will be empty.
  4. Drag and drop the Scatter with Regression Plot (which contains one of your regression curves to be overlaid) by its border onto the Scatter Plot you created in Step 3 to make an overlay. Choose "2D Plot Overlay(s)" in the Paste format dialog that appears and click OK.
  5. Repeat Step 4 for each additional desired Scatter with Regression plot to be overlaid onto the Scatter plot. You should now have a plot with multiple Scatter with Regression curves. If necessary, you may Format the plot axes, title, and legend as usual.

Overlaid Data Points

By creating a Scatter Plot of your reference data points and using it as your base overlay, your Scatter with Regression curve(s) may be visualized as overlays on the Scatter Plot. This is useful for plotting interpolated concentrations, theoretical reference values, etc.

All plots and Spreadsheets referred to below must be visible on the same page of your layout.

  1. Create one Scatter with Regression Plot for each regression curve that you need.
  2. Insert a new, empty Spreadsheet (Insert tab> Spreadsheet> New Spreadsheet). Enter X and Y coordinates for the reference value(s) in successive columns. If you will plot multiple reference values, each should be in its own row.
  3. Insert a Scatter Plot (not a Scatter with Regression Plot), while the new Spreadsheet is selected. The Scatter Plot will contain the reference values..
  4. Drag and drop the Scatter with Regression Plot (which contains one of your regression curves to be overlaid) by its border onto the Scatter Plot you created in Step 3 to make an overlay. Choose "2D Plot Overlay(s)" in the Paste format dialog that appears and click OK.
  5. Repeat Step 4 for each additional desired Scatter with Regression plot to be overlaid onto the Scatter plot. You should now have a plot with your Scatter with Regression curve(s) and your reference value(s) depcited as points (e.g., the blue "Amount of Ab to use" point in the Titration plot below). If necessary, you may Format the plot axes, title, and legend as usual.
  6. Also see our Antibody Titration use case on Scatter with Regression plots.