FCS Express: Spectral Data Analysis

Spectral flow cytometry is used to sample more parameters per single cell or particle than traditional flow cytometry. In contrast to conventional flow cytometers, spectral flow analyzers and sorters substitute mirrors, optical filters, and photomultiplier tubes (PMTs) with a spectrograph and multichannel detector to use fluorescence or Raman spectroscopy.

FCS Express 6 now introduces the capabilty to read raw spectral data from Sony instruments stored with the .SRAW file format and a new 1D plot type - Spectrum Plots for analysis the spectral data. Data can be presented in the spectrum plot with numerous density, line, and backgating options to optimize displays for analysis. The color of each line in can reflect the color of the gate in which the event falls effectively providing backgating information for spectral data. Multiple populations or files may also be overlaid with ease.

For more details on using Spectrum plots please see the Spectrum Analysis section of the FCS Express manual.

Spectrum plots allow for analysis of spectral data at the cell by cell spectra or averaged level. Spectral plots may easily be gated and overlaid.
Spectrum lines may be colored according to the gate that they are associated with to allow "backgating".