FCS Express: Nexcelom Applications and Layouts

Nexcelom

The Nexcelom Cellometer Vision CBA Analysis System allows the user to quickly acquire imaging data for a variety of standard assays. Your multi parameter imaging data can now be easily analyzed in FCS Express and expanded upon using all of the tools and features FCS Express has to offer. Please visit our Nexcelom Partnership page to learn more about this exciting collaboration, view the recorded Joint Webinar with Nexcelom and De Novo Software Presentations, or see some applications example videos on the Nexcelom website.

Intro to FCS Express Image Cytometry Video

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Installation and Claiming Instructions

Every purchase of the CBA Vision instrument comes with 2 licenses of FCS Express. A countercode license that is locked to the vision instrument PC and a portable dongle license that you may use on any PC. To get started with your licenses you must first claim and configure them to your own user account. Detailed instructions for the claiming and configuration process are available in our Customer Claim and Configuration Document.

Analysis Layouts

Click the links below to download some of the standard assay layouts for the Nexcelom Cellometer Vision CBA Analysis System. If you do not already have FCS Express installed on your computer, please download and install the free FCS Express Reader before clicking on the links.

Application Layout Download Link
Apoptosis/Necrosis Download Apoptosis/Necrosis Layout
Cell Cycle Download Cell Cycle Layout
Fluorescent Protein Expression Download Fluorescent Protein Expression Layout
Fluorescent Protein Viability Download Fluorescent Protein Viability Layout
Surface Markers Download Surface Markers Layout

(Note: If you have a purchased copy of FCS Express, you can add your .NXDAT files to the Data List in any of the layouts above in order to view your own data)

 

Application Examples

Apoptosis Assay

Download the PDF version of the layout: Nexcelom Apoptosis Assay PDF or download and view the layout using the free FCS Express Reader: Nexcelom Apoptosis Assay.

Assay Information:

Cellometer Vision was developed to count total cells and identify FITC-annexin-V positive apoptotic cells based on the microscopic and fluorescence characteristics of the cell sample. Detection typically takes less than 60 seconds and only requires 20µl of cell sample. Cell images and all analysis data, including cell size distribution histograms, can be saved for documentation. Data can also be easily exported to FCS Express 4 Image Cytometry for further analysis.

Cell Cycle

Download the PDF version of the layout: Nexcelom Cell Cycle Assay PDF or download and view the layout using the free FCS Express Reader: Nexcelom Cell Cycle Assay.

Fluorescent Protein Expression

Download the PDF version of the layout: Nexcelom Fluorescent Protein Expression PDF or download and view the layout using the free FCS Express Reader: Nexcelom Fluorescent Protein Expression.

Assay Information:

Cellometer Vision addresses the need for simple, rapid fluorescence cell counting. By capturing both brightfield and fluorescent images from the same cell sample, Vision can determine the total cell concentration and analyze how many of those cells are expressing GFP. Therefore, using only 20µl of cell sample, researchers can now rapidly identify fluorescence positive cells from a sample, analyze individual cell fluorescence intensity, calculate cell concentration, size and determine the GFP transfection rate in minutes on the lab bench.

Fluorescent Protein and Viability

Download the PDF version of the layout: Nexcelom Fluorescent Protein Viability Assay PDF or download and view the layout using the free FCS Express Reader: Nexcelom Fluorescent Protein Viability Assay.

Assay Information:

Cell viability is routinely tested in cell culture laboratories and used in many assays such as cell proliferation, cytotoxicity, and tumor killing activity. Reliable and accurate viability testing can also be done using propidium iodide (PI), a fluorescent dye. Cellometer Vision was developed to automatically count total cells and propidium iodide fluorescence labeled dead/dying cells for fast, simple PI viability determination.

Surface Markers

Download the PDF version of the layout: Nexcelom Surface Markers Assay PDF or download and view the layout using the free FCS Express Reader: Nexcelom Surface MarkerAssay.