Join the many scientists who have published using FCS Express Image or Plus. Here is a partial list:
- DM Titmarsh, et al. (2016). Induction of Human iPSC-Derived Cardiomyocyte Proliferation Revealed by Combinatorial Screening in High Density Microbioreactor Arrays. Scientific Reports 6:24637.
- D. Kuksin., et al. (2016). Cellometer image cytometry as a complementary tool to flow cytometry for verifying gated cell populations. Analytical Biochemistry 503: 1-7.
- Bethany A. Rhein, Linda S., et. al. (2015) Interferon-γ Inhibits Ebola Virus Infection. PLoS Patholg. Nov 12, 2015; 11(11):e1005263.
- Srinivas, S., et al (2015). A Novel Method for Assessment of Natural Killer Cell Cytotoxicity Using Image Cytometry. PLOS ONE. 10(10):e01412074.
- Yasuteru, S., et al (2015). Two-pore channels control Ebola virus host cell entry and are drug targets for disease treatment Science. 347(6225): 995-998.
- Roukos, V., et al (2015). Cell cycle staging of individual cells by fluorescence microscopyNature Protocols. 10:334-348.
- Mulligan,S.K., et al (2015). Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles.Microscopy and Microanalysis. 21:1017.
- McDonagh, P., et al.(2015). Combination siRNA therapy against feline coronavirus can delay the emergence of antiviral resistance in vitro. Veterinary Microbiology. 176:10-18.
- Gorman, B. R., et al. (2014).Multi-Scale Imaging and Informatics Pipeline for In Situ Pluripotent Stem Cell Analysis. PLoS One. 9(12): e116037.
- Etzerodt A., et al. (2014).Structural Basis for Inflammation-driven Shedding of CD163 Ectodomain and Tumor Necrosis Factor-α in Macrophages. Indian Journ of Med Research.J Biol Chem. 289(2):778-88.
- Wang J., et al. (2014).Evaluation of the antitumor effects of c-Myc-Max heterodimerization inhibitor 100258-F4 in ovarian cancer cells. Indian Journ of Med Research. J Transl Med. 12: 226.
- McDonagh, P., et al. (2014).Identification and characterisation of small molecule inhibitors of feline coronavirus replication. Veterinary Microbiology 174(3-4):438-447.
High Content Analysis of a Robust 384-Well Cell Migration Assay
Sean Burke1, Tim Baranowski2, Jennifer A. Fronczak3, Jayne Hesley2, Keren Hulkower3, and David Novo1
1De Novo Software, Inc. Los Angeles, CA, 2Molecular Devices, Inc. Sunnyvale, CA, 3Platypus Technologies, Madison, Wisconsin
Presented at CYTO 2011
Quantifying cell migration in an objective manner is of interest to researchers studying cancer metastasis and wound healing. The Oris™ Pro 384 Cell Migration Assay (Platypus Technologies) is a first-in-class, automatable, 384- well high throughput screening assay. By using a novel approach to image analysis, combining the ImageXpress® Velos laser scanning cytometer (Molecular Devices) and FCS Express 4 Image Cytometry™ (De Novo Software), we were able to rapidly analyze the effects of cell seeding density and inhibitors on the motility of three different cell lines.
(Click image to download)
Homogeneous Multiplexed Assay for Hematopoietic Stem Cell Toxicity
Oksana Sirenko1, Pierre Turpin1, George Klarmann3,Jayne Hesley1, Sean Burke2, Michael Sjaastad2, David Novo2, H. Roger Tang1, and Evan F. Cromwell1
1Molecular Devices, Inc. Sunnyvale, CA, 2De Novo Software, Inc. Los Angeles, CA, 3Lonza, Inc. Walkersville, MD
Presented at Lab Automation 2011
Many potential pharmaceuticals fail in clinical trials due to unacceptable toxicity, thus early discovery and elimination of toxic compounds is important to the drug discovery process. For example, in chemotherapy toxicity to hematopoietic stem cells (HSC) or bone marrow often limits dosing and duration and can cause anemia, neutropenia, or other side effects. One way to improve information on toxicity is to introduce more biologically relevant assays early in development. Hematopoietic progenitors are extremely sensitive to the toxic side effects of many compounds and can provide valuable information about both general and lineage-specific toxicity making them useful for screening of therapeutics for cancers and other diseases. Studies have shown that the inhibitory activity of several drugs, as measured in in vitro assays of hematopoietic progenitor differentiation, correlate with their in vivo activities in various animal models (2, 3). Toxic effects of drugs can also be lineage-specific (4, 5). However, primary cells, especially non-adherent hematopoietic progenitors, are more challenging to use in automated high-throughput assays compared to adherent cell lines. Current HSC assays using flow cytometry are labor intensive and less amenable to high-throughput. Here we report on the use of IsoCyte cytometer (Molecular Devices, Inc.) and FCS Express® 4 Image Cytometry software (De Novo Software) to automate the study of cell differentiation, cytotoxicity, and carcinogenesis in 96 or 384 multi-well plate formats at throughputs of 3-5 minutes per plate.
(Click image to download)
Please Contact Us if you have published data using FCS Express Image Cytometry and would like to appear here!