FCS Express: Image Cytometry Application Examples

Image Cytometry Applications Examples

FCS Express is one of the premier tools for analysis and reporting of your image cytometry data. The application examples below illustrate how FCS Express can be used to create stunning reports while easily performing complex analysis. Jump to Flow Cytometry Applications.

Would you like to see your Image Cytometry Application in this list? If so please email us at support@denovosoftware.com.

Immune Synapse Analysis and Reporting from Amnis Data

FCS Express allows you to work directly with your Amnis derived data sets in their native .daf / .cif format. Complex analysis routines and workflows are made easy with FCS Express. In the Immune Synapse example see how a complex gating strategy is quickly created, displayed, and reported across a seven point drug titration to create an IC50 curve that updates in real time as gates change.. See for yourself by exploring the layout using the free FCS Express Reader: Immune Synapse Analysis.

Apoptosis Assay via the Cellometer® Vision by Nexcelom

The Nexcelom Cellometer Vision allows the user to quickly acquire imaging data for a variety of standard assays including Apoptosis. Your multi parameter imaging data can now be easily analyzed in FCS Express and expanded upon using all of the tools and features FCS Express has to offer. See for yourself by downloading the PDF version of the layout: Nexcelom Apoptosis Assay PDF or by using the free FCS Express Reader: Nexcelom Apoptosis Assay. Please visit our Nexcelom Partnership page to learn more about this exciting collaboration.

See a full list of Nexcelom Application Examples and Layout downloads

In-Situ Rat Cerebellum Image Analysis with CellProfiler Segmentation

You have carefully planned, implemented, and imaged your multi color in-situ staining experiment resulting in large montaged images containing tens or even hundreds of thousands of cells...now what? After segmentation with CellProfiler, FCS Express can handle these large data sets much like a multi color flow cytometry experiment but with the added advantage of spacial resolution and the ability quickly move from the population level down to individual cells and images. See for yourself by exploring the layout using the free FCS Express Reader: In Situ Rat Cerebellum Staining or download a PDF version: In Situ Rat Cerebellum Staining. You may also download the sample data and run the pipeline yourself using the In Situ Rat Cerebellum Staining Sample Data and pipeline.

Images courtesy of Dragan Maric, Ph.D.

Cell Cycle Image Cytometry Assay via the Countstar

The Ruiyu Countstar Fluorescence Cell Analyzer allows the user to quickly acquire imaging data for cell cycle analysis in FCS Express while retaining links between data points and images. See how easy it is by downloading the PDF version of the layout: Countstar Cell Cycle Analysis PDF or by using the free FCS Express Reader: Countstar Cell Cycle Assay. Please visit our RuiYu Biotech Partnership page to learn more about this exciting collaboration.

Working with Amnis IDEAS Derived Data in FCS Express

Data from the Amnis IDEAS software package can be exported for use in FCS Express. Use FCS Express to navigate between plots and images in real time, create custom statistics, and export directly to PowerPoint for quick and easy presentation of your data.

See for yourself while learning FCS Express Image Cytometry exploring the layout using the free FCS Express Reader: Image Cytometry Data in FCS Express or download a PowerPoint version: Image Cytometry Data in FCS Express PowerPoint. Please visit our Amnis knowledge base page to learn more about exporting your data from IDEAS Software for use in FCS Express Image Cytometry.

Cell Cycle Analysis of Amnis IDEAS Image Cytometry Data

DNA Cell Cycle analysis and modeling is easy to perform with Amnis IDEAS derived data sets in FCS Express Image Cytometry. Use Multicycle to generate powerful cell cycle statistics and batch process your image cytometry data to analyze and archive your results in record time. See for yourself by exploring the layout using the free FCS Express Reader: Amnis (IDEAS) Analysis with Multicycle in FCS Express Image or download a PDF version: Amnis (IDEAS) DNA Analysis PDF. Please visit our Amnis knowledge base page to learn more about exporting your data from IDEAS Software for use in FCS Express Image Cytometry.

High Throughput Dose Response Assay with the Thermo Scientific Cellomics Platform

Cellomics best-in-class technology integrates automated instrumentation, image analysis modules, multi-parametric assays, validated reagents and cell lines and cellular bioinformatics tools to achieve a flexible, broadly applicable platform for users in the life sciences who need to understand cell function. FCS Express Image Cytometry compliments these systems by providing easy to use software with robust reporting, statistical, and batch processing features. FCS Express works at the single cell level and dose response curves update dynamically as gates are adjusted. See for yourself while learning FCS Express Image Cytometry with our Introduction to FCS Express Image Cytometry tutorial. You may also explore the layout using the free FCS Express Reader: Cellomics Dose Response Assay or download a PDF version: Cellomics Dose Response PDF.

Life Technologies Tali Cell Cycle Analysis

Download the PDF version of the layout: Tali Cell Cycle Assay PDF or download and view the layout using the free FCS Express Reader: Tali Cell Cycle Assay.

Assay Information:

The Tali Cell Cycle Kit allows you to easily stain and identify the cell cycle in a number of cell lines. The Tali data can then be exported to FCS Express Image Cytometry to analyze with the Multicycle cell cycle analysis plug-in for FCS Express. By utilizing FCS Express for cell cycle modeling you can quickly and easily get an accurate and powerful break down your cell cycle populations and use statistics provided by FCS Express to dive further into your analysis.

High Content Assay with PerkinElmer's Opera HCS System

Incorporating industry-leading optical technologies, PerkinElmer's Opera® High Content Screening System continues to be the world's most proven confocal microplate imaging solution for high throughput High Content Screening. FCS Express Image Cytometry compliments these systems by providing easy to use software with robust reporting, statistical, and batch processing features. FCS Express works at the single cell level and dose response curves update dynamically as gates are adjusted. See for yourself while learning FCS Express Image Cytometry with our Introduction to FCS Express Image Cytometry tutorial. You can also take a closer look by downloading the Opera Dose Response Powerpoint export for this experiment.

Multiwell plate image analysis with Image J Segmentation

Multiwell plate data processed in ImageJ can be exported using ImageJ macros from De Novo Software and analyzed with FCS Express. You may also explore the layout using the free FCS Express Reader: Image J Analysis with FCS Express or download the PDF version: ImageJ Research Imaging PDF.

Phase Holographic Imaging

Phase Holographic Imaging now enables exporting Image Cytometry Experiment files from the HStudio software. The holographic images and derived numerical data acquired on the HoloMonitor instrument can now be analyzed in FCS Express. See what holographic imaging can do by using the free FCS Express Reader to open: Phase Holographic Imaging Data or download the PDF version: Phase Holographic Imaging Data in FCS Express.

Single Image analysis with CellProfiler Segmentation

FCS Express can be used to organize, analyze, and get results from any imaging experiment. By using CellProfiler to segment your images you can analyze the simplest single image experiments to complex multi-time point and multi-color assays in FCS Express. See for yourself while learning FCS Express Image Cytometry with our CellProfiler Export tutorial. You may also explore the layout using the free FCS Express Reader: Nuclear Translocation Assay with CellProfiler Segmentation or download a PDF version: High Content Research Imaging PDF and Research Imaging PDF

Single Image analysis with Image J Segmentation

FCS Express can also be used in conjunction with ImageJ to get data from your images. By using ImageJ to segment your images and macros from De Novo Software you can quickly export and analyze your data with FCS Express. See for yourself while learning FCS Express Image Cytometry with our ImageJ Export tutorial. You may also explore the layout using the free FCS Express Reader: Image J Analysis with FCS Express or download a PDF version: ImageJ Research Imaging PDF.

Western Blot and Imaging Data Combined

Explore the layout using the free FCS Express Reader: Western Blot and Image Analysis with CellProfiler Segmentation or download a PowerPoint version: Western Blot and Imaging PPT.

Transmission Electron Microscopy in FCS Express

p> cells and gold labeled antibodies a complete analysis of your micrographs can be completed. Explore the layout using the free FCS Express Reader: TEM Analysis with FCS Express or download a PowerPoint version: TEM Analysis PPT Export.

Histology core section analysis with CellProfiler Segmentation

Generating large data sets to look at multiple tumor core histology sections has become relatively easy with new imaging technology although bottle necks usually occur during the analysis step. Now with FCS Express Image Cytometry making sense of the data and analyzing it quickly is made easy with the use of interactive plots and batch analysis. See for yourself by exploring the layout using the free FCS Express Reader: Histo Core Analysis or download a PDF version: Histo Core Analysis. You may also download the sample data and run the pipeline yourself using the Histo Core Analysis Sample Data and pipeline.

Images courtesy of Michael Samuel, Ph.D.

Tissue section analysis with Image J Segmentation

Histological sections with tiled images (i.e., contiguous fields of view) which have been segmented in ImageJ can be exported and stitched together in FCS Express, using ImageJ macros from De Novo Software. You may explore the layout using the free FCS Express Reader: Image J Analysis of Tiled Images with FCS Express or download the PDF version: ImageJ Tiled Images PDF.

Live-Dead Assay with Molecular Devices IsoCyte/ImageXpress™ Velos

The ImageXpress Velos allows you to image and segment entire plates (6 to 1536-well) in less than 5 minutes. FCS Express Image Cytometry now supports Velos data and complements your high speed analysis with in depth multiparametric capabilities. Try for yourself while learning FCS Express with our Exporting from IX Velos tutorial. You may also explore the layout using the free FCS Express Reader: IX Velos Data Analysis in FCS Express Image or download a PDF version: IX Velos Data Analysis PDF.

Colony Counting with CellProfiler Segmentation

Colony counting can be a tedious task at best. Let FCS Express Image Cytometry do the counting and classification for you so you can spend more time getting to results. Explore the layout using the free FCS Express Reader: Colony Counting in FCS Express or download a PDF version: Colony Counting PDF. You may also download and run the pipeline yourself using the CellProfiler colony counting pipeline adapted for FCS Express.

Chloroplast Analysis with the Etaluma LumaScope™ and CellProfiler Segmentation

Etaluma Inc., maker of the LumaScope, is focused on bringing analytical and diagnostic instrumentation to a greater spectrum of users. The LumaScope™ provides fluorescence and bright field capability in an inexpensive inverted microscope and when images are segmented by CellProfiler single cells and objects can easily be analyzed in FCS Express Image Cytometry. See for yourself by downloading the PDF version of the Chloroplast Analysis layout, or by using the free FCS Express Reader: Chloroplast Analysis.

In the Chloroplast Analysis example a bright field and fluorescent image were taken to highlight the distribution of the Chloroplast. FCS Express enables the user to quickly count and categorize chloroplasts in the image.

Cell Cycle using Image Cytometry analysis via the Chemometec NucleoCounter® NC-3000™

The Chemometec NucleoCounter makes acquiring DNA content data from slides quick and easy. FCS Express Image Cytometry now makes analysis of your images and getting to results just as simple. Use Multicycle to generate powerful cell cycle statistics and batch process your image cytometry data to analyze and archive your results in record time. See for yourself by exploring the layout using the free FCS Express Reader: Chemometec Analysis with Multicycle in FCS Express Image or download a PDF version: Chemometec DNA Analysis PDF. Please visit our knowledge base page to learn more about exporting your data from the NucleoView NC-3000 Software for use in FCS Express Image. Cytometry or our Chemometec Partnership page to learn more about this collaboration.

See a full list of Chemometec Application Examples and Layout downloads

 

Would you like to see your Image Cytometry Application in this list? If so please email us at support@denovosoftware.com.

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Publications Using FCS Express Image Cytometry

 

 

NucleoCounter is a registered trademark of Chemometec.

NC-3000 is a trademark of Chemometec.

Cellometer is a registered trademark of Nexcelom.

Opera is a registered trademark of PerkinElmer