FCS Express: High Content Image Analysis

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FCS Express continues to lead the field by providing cutting edge high content visualization tools. FCS Express supports heat map plots for displaying plate based, high content screening, high content analysis, image analysis data. Data for heat maps can be generated from many manufacturers such as Cell Profiler, Nexcelom Celigo, Thermo ArrayScan, and more Image Data Format Compatibility is being added all the time.


Heat maps allow the user to:

  • Plot any statistic on any parameter.
  • Represent different files, statistics, or parameters simultaneously with multiple Heat Maps, Heat Map Overlays, or a Heat Map Radius (right). 

  • Apply any kind of gate to Heat Maps, including well gates; wells can then be individually examined as any other type of plot that FCS Express supports.
  • Work with High Content Screening or High Content Analysis data sets.
  • Complete control over plate formatting and displays.

Gates can easily be created on any heatmap through the Gating Tab (below-top row) or by right clicking on a plot and choosing Create Gate>Well (bottom-right). Gates created on a heat map can be applied to dot plots, histograms, etc., just like any other gate. The video below also shows heat map gating in more detail or the full webinar on working with heat maps in FCS Express may be viewed.

Heat Map Gating

Any parameter or statistic associated with your heat map can be displayed on the axis of the plot or used for subsequent 1D or 2D plotting.

Parameters can be chosen from the formatting window....

Or parameters can be chosen by right clicking on the axis of the heat map





Just like any other plot in FCS Express, Heat Maps are fully formattable: choose from several color schemes, and represent different files, statistics, or parameters simultaneously as Heat Map Overlays and/or Radius, as shown below. Click on the images below to see a larger image.

Color Schemes

Green - Blue
Green - Red
Rainbow

Threshold
(a user-defined threshold highlights wells that fall above or below a value)

Grayscale
Inverse Grayscale
Gradient
(Red, Green, or Blue)

Displaying Additional Data, Parameters, or Statistics

Overlays (up to six)
Radius
Overlays + Radius