FCS Express: High Content Screening

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High Content Tools for Flow Cytometry and Image Cytometry

FCS Express supports heat map plots for displaying plate based, high content screening, high content analysis, image and flow cytometry data.

 

High Content Imaging

Heat maps allow the user to:

  • Plot any statistic on any parameter.
  • Plot multiple heat maps from different files, statistics, or parameters simultaneously. 
  • Heat maps can be gated on any gate you like, including by well, and wells can then be individually examined as any other type of plot that FCS Express supports including the new Picture Plot feature.

Gates can easily be created on any heatmap through the Gating Tab (below-top row) or by right clicking on a plot and choosing Create Gate>Well (bottom-right). Gates created on a heat map can be applied to dot plots, histogram etc, just like any other gate. The video (bottom-left) also shows heat map gating in more detail.

Heat Map Gating

 

Any parameter or statistic associated with your heat map can be displayed on the axis of the plot or used for subsequent 1D or 2D plotting.

Parameters can be chosen from the formatting window.... Or parameters can be chosen by clicking on the Y axis of the heat map.




Just like any other plot in FCS Express, Heat Maps are fully formattable, including the six different plate color schemes seen below.

 

Green-Blue Rainbow Red-Black

Grey scale

Inverse Grey scale Threshold-set a user defined threshold to highlight wells that fall above or below a value.

 

High Content Flow Cytometry

FCS Express continues a legacy of innovation in the field by providing analysis capabilities for 96 well plate format flow cytometry data from the following instruments:

  • BD HTS
  • Guava
  • Coulter Quanta
  • Hypercyte
  • Sony
  • Accuri
  • MACSQuant

The imported data can be displayed on a per well basis or as an entire plate in Heat Map format.

Heat maps allow the user to:

  • Plot any statistic on any parameter.
  • Plot multiple Heat Maps from different files, statistics, or parameters simultaneously. 
  • Heat Maps can be gated on any gate you like, including by well, and wells can then be individually examined as any other type of plot that FCS Express supports.
  • Work with High Content Screening or High Content Analysis data sets

Gates can easily be created on any heatmap through the Gating Tab (below-top row) or by right clicking on a plot and choosing Create Gate>Well (bottom-right). Gates created on a heat map can be applied to dot plots, histogram etc, just like any other gate.

Heat Map Gating

Any parameter or statistic associated with your Heat Map can be displayed on the axis of the plot.

Parameters can be chosen by right clicking on the axis of the Heat Map


Just like any other plot in FCS Express, Heat Maps are fully formattable, including the six different plate color schemes seen below. Click on the images below to see a larger image.

Green-Blue Rainbow Red-Black Grey scale
Inverse Grey scale Threshold